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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 目录编号:
209-402-241
- 库存:
详询
- 供应商:
广州市左克生物
- 浓度:
见包装
- 抗体英文名:
40 nm Colloidal Gold IgG Fraction Monoclonal Mouse Anti-Human IgE (ME.114)
- 规格:
1ml
产品简介:
品牌:Jacksonimmuno
货号:209-402-241
产品名称:40 nm Colloidal Gold IgG Fraction Monoclonal Mouse Anti-Human IgE (ME.114)
https://www.jacksonimmuno.com/catalog/products/209-402-241
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文献和实验buffer (pH9.0) ~ 200 ml. Filter protein mixture Add protein mixture or culture supernatant containing Ab (adjust pH to 7.8 with Binding buffer ; red color) to the Protein A column. Mouse antibodies of the IgG1 subclass
【翻译】Development trends for monoclonal antibody cancer therapeutics
7 and patients frequently developed antibodies to the mouse-derived proteins, which limited their utility8, 9. In addition, only certain murine mAb isotypes have been shown to effectively bind to and activate elements of the human immune
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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