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- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 目录编号:
209-065-098
- 库存:
详询
- 供应商:
广州市左克生物
- 浓度:
见包装
- 抗体英文名:
Biotin-SP-conjugated AffiniPure® Mouse Anti-Human IgG, Fcγ Fragment Specific (min X Bov,Hrs,Ms Sr Prot)
- 规格:
1ml
产品简介:
品牌:Jacksonimmuno
货号:209-065-098
产品名称:Biotin-SP-conjugated AffiniPure® Mouse Anti-Human IgG, Fcγ Fragment Specific (min X Bov,Hrs,Ms Sr Prot)
https://www.jacksonimmuno.com/catalog/products/209-065-098
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文献和实验Site-Specific Chemical Modification of a Glycoprotein Fragment Expressed in Yeast
secretory pathway. The N-terminal cysteine is then conjugated with a biotin thioester to produce a biotinylated, glycosylated IgG1 Fc using native chemical ligation.
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
. A cheap alternative is to exploit the relative lower levels of each IgG isotype in serum and on follicular B cells to stain with isotype-specific, conjugated, anti-mouse secondary antibodies. IgG isotypes account each for 20-25% ot total serum
by a two- or three-step procedure. The method can insert any fragment into small or large episomes, without the need of an antibiotic selection gene. We have humanized the mouse luteinizing hormone receptor gene (Lhcgr ) by inserting a ~55 kb fragment from a BAC clone
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