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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 目录编号:
112-655-071
- 库存:
详询
- 供应商:
广州市左克生物
- 浓度:
见包装
- 抗体英文名:
Alexa Fluor® 790-conjugated AffiniPure® Goat Anti-Rat IgG, Fcγ Fragment Specific (min X Hu,Bov,Hrs Sr Prot)
- 规格:
0.5mg
产品简介:
品牌:Jacksonimmuno
货号:112-655-071
产品名称:Alexa Fluor® 790-conjugated AffiniPure® Goat Anti-Rat IgG, Fcγ Fragment Specific (min X Hu,Bov,Hrs Sr Prot)
https://www.jacksonimmuno.com/catalog/products/112-655-071
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文献和实验Site-Specific Chemical Modification of a Glycoprotein Fragment Expressed in Yeast
immunoglobulin G subclass 1 fragment crystallizable (IgG1 Fc), by native chemical ligation. The glycosylated IgG1 Fc is expressed in a glycosylation-deficient yeast strain. The N-terminal cysteine is generated by the endogenous yeast protease Kex2 in the yeast
偶联物,最大限度的提高了信噪比。图2. CF染料的超强特异性;由于AF染料制备过程中含有大量带负电的磺化基团,容易非特异性结合正电基团,请看AF790 Dye与CF790的条带特异性对比。3. 优异的标记效率生物偶联后的活性染料一般很容易水解,会在运输,操作及储存中带来诸多不便,最终致染料结合效率低下。像Alexa Fluor® dyes,DyLight? dyes and IRDyes®等严重的磺化作用有较强的吸湿性,恶化水解作用等问题。例如: AlexaFluor® 488 在应用时琥珀酰亚胺脂酯
Technovit® 9100 Methyl Methacrylate
with buffer (see above) DAKO EnVision polyvalent antibody (goat-anti-mouse/goat-anti-rabbit) coupled to alkaline phosphatase 30 min at room temperature Visualization Step
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