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- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 目录编号:
108-055-008
- 库存:
详询
- 供应商:
广州市左克生物
- 浓度:
见包装
- 抗体英文名:
Alkaline Phosphatase-conjugated AffiniPure® Goat Anti-Horse IgG, Fc Fragment Specific
- 规格:
1ml
产品简介:
品牌:Jacksonimmuno
货号:108-055-008
产品名称:Alkaline Phosphatase-conjugated AffiniPure® Goat Anti-Horse IgG, Fc Fragment Specific
https://www.jacksonimmuno.com/catalog/products/108-055-008
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文献和实验and wash the blot 3 times, 5-7 minutes each, with 0.5% BSA in TBST.11. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection
each, with 0.5% BSA in TBST. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection which employs goat anti
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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