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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 目录编号:
015-000-003
- 库存:
详询
- 供应商:
广州市左克生物
- 浓度:
见包装
- 抗体英文名:
ChromPure™ Mouse IgG, whole molecule
- 规格:
5mg
产品简介:
品牌:Jacksonimmuno
货号:015-000-003
产品名称:ChromPure™ Mouse IgG, whole molecule
https://www.jacksonimmuno.com/catalog/products/015-000-003
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文献和实验过程和troubleshoot 试剂盒规格: 22 ChIP操作 试剂盒组成 * DNA Purification Spin Columns and Collection Tubes * Protein G Agarose / Salmon Sperm DNA * Anti-RNA Polymerase II,clone CTD448 * Control PCR Primers * Normal Mouse IgG * Bind,Wash
Mouse Models of Autoimmune Blistering Diseases Induced by the Passive Transfer of Antibodies
Passive transfer of IgG into neonatal mice is a potential method of reproducing antibody-mediated blistering skin diseases. The major autoantigen for bullous pemphigoid is collagen XVII (COL17)/BP180, which is an epidermal linker
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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