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- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 目录编号:
005-060-003
- 库存:
详询
- 供应商:
广州市左克生物
- 浓度:
见包装
- 抗体英文名:
Biotin-SP-conjugated ChromPure™ Goat IgG, whole molecule
- 规格:
1mg
产品简介:
品牌:Jacksonimmuno
货号:005-060-003
产品名称:Biotin-SP-conjugated ChromPure™ Goat IgG, whole molecule
https://www.jacksonimmuno.com/catalog/products/005-060-003
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文献和实验. We have developed a novel technique for triple-labeling immunofluorescence that can be used with primary antibodies derived from a single host source. This technique includes modification of one primary antibody with biotin (ChromaLink™ Biotin) and a second primary
by staining in simple IHC the relevant heterologous primary antibodies. E.g. many goat anti mouse IgG1 heavy chain do stain rat IgG2a and IgG2b . Procedure: First stain HRP Developing solution Second stain AP
to a goat antimouse IgG, which catalyzes the substrate p-nitrophenyl phosphate, to form a colored product. Excess reagents are removed by washing, and enzyme activity can be measured with an ELISA plate reader at 415nm. The amount of colored product
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