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- 详细信息
- 文献和实验
- 技术资料
- 库存:
119
- 英文名:
pCDNA3.1-OspF-HA
- 保质期:
2年
- 供应商:
上海烜雅生物科技有限公司
- 保存条件:
2-8℃
- 规格:
少量干粉质粒/大提液体质粒
| 规格: | 少量干粉质粒 | 产品价格: | ¥480.0 |
|---|---|---|---|
| 规格: | 大提液体质粒 | 产品价格: | ¥680.0 |
别称:
基因长:720bp
质粒宿主:哺乳细胞
质粒用途:蛋白表达
片段类型:ORF
片段物种:
原核抗性:Amp
筛选标记:Neo/G418
荧光标记:
启动子:CMV
复制子:pUC
感受态:DH5a
温度:37度
正向引物:CMV-F:CGCAAATGGGCGGTAGGCGTG
反向引物:BGH:TAGAAGGCACAGTCGAGG
注意事项:本产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药。
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文献和实验Recombinant DNA Engineering, Or Cloning Genes In Plasmids
the plasmid DNA with very high fidelity. Plasmid DNA is physically independent of genomic DNA and this allows for their easy isolation from bacterial cultures. the pcDNA3.1+ plasmidVector plasmids used for cloning are short, circular, double-stranded DNA
Hi guys, I have a question for you. I made a construct which had been sequenced. It is in pcDNA3.1(+) and with HA-TAA to stop. The right size for endogenous protein is 31kD. But when I transfected into COS-7 and run western, the size
Detection of ERK1/2 Activities Using Affinity Reagents
-7 cells were transfected with pCDNA3-HA-ERK2 or pECE-HA-MAPK (HA-p38α) in six-well plates using the DEAE-dextran method (1 μg of DNA/well). Cells were activated with 50 ng/mL of EGF for 5 min (for HA-ERK2; lanes 1 and 2) or with 0.5 M sorbitol for 20 min
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