(Cyclobutyl-piperazine x 2 HCl

2

) 1. Add 292 g acrylamide and 8 g piperazine diacrylamide (PDA) to 700 ml HPLC-grade H2O, under the hood . 2. Stir to dissolve. Bring volume to 1 L . 3. Filter through 0.45 µm pore size filter. Store at 4°C in the dark. D

2-D Polyacrylamide Gel Electrophoresis

1.Add 300 g Tris-base,1441 g glycine,and 10 g SDS to ~7 L HPLC-grade H2O. 2.Mix gently until dissolved. 3.Bring volume to 10 liters. C: 30% Acrylamide Stock (1 liter) 1.Add 292 g acrylamide and 8 g piperazine diacrylamide (PDA)to 700 ml HPLC-grade H2

大叶紫株中乌索酸的提取纯化与反相高效液相色谱分析

%。结论“醇提凝析法”提取乌索酸工艺实用可行，为工业试验生产乌索酸提供了技术条件；RP-HPLC法测定乌索酸含量准确、精密度高、重复性好、线性范围宽，可作为控制乌索酸质量的方法。 　　【关键词】  大叶紫株叶 乌索酸 结构表征 反相高效液相色谱 　　Abstract：ObjectiveTo extract and purify ursolic acid from Callicarpa macrophylla Vahl leaves and to establish