(3-BROMOQUINOLIN-6-YL)ACETATE

Two‐Step, One‐Pot Synthesis of Inosine, Guanosine, and 2′‐Deoxyguanosine O6‐Ethers via Intermediate O6‐(Benzotriazol‐1‐yl) Derivatives

(Spectrum) 200‐ to 300‐mesh silica gel (Natland, http://www.natland.com/) Ethyl acetate (EtOAc), HPLC grade (Fisher Scientific

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

Tetra‐n ‐butylammonium fluoride (TBAF; Aldrich) Tetrahydrofuran (THF; Acros) HPLC buffer A: 0.1 M triethylammonium acetate

祖卡木颗粒中罂粟碱含量的反相高效液相色谱法测定

　　【摘要】 　　目的采用反相高效液相色谱（RP�HPLC）法测定祖卡木颗粒中罂粟碱的含量。方法使用DIKMA C8色谱柱（250 mm×4.6 mm，5μm），甲醇�乙腈�1%三乙胺�1%乙酸铵（40∶20∶1∶39）为流动相，流速1 ml/min，检测波长为240 nm。结果罂粟碱在0.019 8～0.396μg范围内呈良好线性关系（r＝0.999 9），平均回收率为99.6%，RSD为1.5%。结论该法灵敏、准确，专属性强，重复性好，可以作为祖卡木颗粒的含量测定方法。