(Tetrahydroisoquinoline-1-carb

Techniques for Measuring the Activity of Carboxylic Acid:CoA Ligase and Acyl‐CoA:Amino Acid N‐Acyltransferase: The Amino Acid Conjugation Pathway

of Carboxylic Acid:CoA Ligase using AMP Formation Coupled to NADH Oxidation Alternate Protocol 4: Monitoring Carboxylic Acid:CoA Ligase Activity by HPLC Determination of Acyl‐CoA Product Formation Basic Protocol

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

of the biotinylated c?di?GMP conjugate, and isolated in similar yields. Curr. Protoc. Nucleic Acid Chem. 52:14.9.1?14.9.20. © 2013 by John Wiley & Sons, Inc. Keywords: propargylated c?di?GMP; biotinylated azide; click conjugation reaction

Synthesis and Use of Pseudopeptides Derived from 1,2,4-Oxadiazole-, 1,3,4-Oxadiazole-, and 1,2,4-Triazole-based Dipeptidomimetics

acids, and the reaction conditions have been chosen so that the enantiopurity is retained during the reaction sequences. Two series of mimetics will be described, one in which the carboxylic acid functionality is directly attached to the heterocyclic