(Potassium4-trifluoroboratomet
hylthiomorpholine 1150654-80-0 **科研现货速达** HPLC 随货提供COA、HPLC谱图、MS谱图，NMR图谱等，详细联系客服。用于LC-MS/MS定量分析，代谢组学、质谱，药代动力学等- 询价
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Potassium4-trifluoroboratomethylthiomorpholine
- 库存：
999
- 供应商：
智溯科ZSK
- CAS号：
1150654-80-0
- 规格：
10mg///100mg///200mg
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文献和实验Microscale Hot Borate RNA Extraction from Cotton Tissue--从棉花组织中提取微量RNA
for 1.5 hours in the 42℃ incubator/shaker.Remove from incubation. Add 1040 µL of 2M potassium chloride (for a final concentration of 160 mM; this step will precipitate proteins from the extract). Gently vortex to mix. Incubate on ice for 1 hour.Centrifuge
Biology Reporter 6(4): 253-257.The key to the success of this protocol rests, I think, with the initial, high molarity potassium acetate ppt. The RNA is rescued from the bad things that ppt out. Also important are the three detergents (cationic, anionic
prior to storage in a -70 to -80 C freezer. Thaw on ice just before using in a transformation experiment. I typically transform 50 ul Cell s with 2-10 ul of a ligation reaction, and you should get between 1x10exp8 to 1x10exp9 cfu"s/ug DNA. Medium
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