(S)-3-Amino-2-hydroxy-propioni

Determination of Sulfur Amino Acids, Glutathione, and Related Aminothiols in Biological Samples by Gas Chromatography with Flame Photometric Detection

. GC-MS methods based on the conversion into tert -butyldimethylsilyl (33 ) and N(O,S)-propoxycarbonyl propyl ester (34 ) derivatives, and LC-MS method were highly sensitive and specific, but these methods require expensive equipment. Furthermore, CZE

运用肽库筛选磷酸化激酶motif方法 Screening Kinase Phosphorylation Motifs Using Peptide Libraries

agarose and methyl esterification, resuspend the sample from step 14 in solution 2. If NTA agarose is used and methyl esterification was not performed, resuspend the sample from step 11 in 50 l of 0.1 M acetic acid, adjust pH to 3.5 with 30% acetic acid

浅析染色质免疫沉淀（ChIP）技术在 DNA 与蛋白质相互作用研究中的重要性

[4] 。由于在信号转导和表观遗传研究中的突出作用，ChIP 在肿瘤 [5-7] 、神经科学 [8-10] 、植物发育 [11-13] 等领域中应用非常广泛，同时有关细胞凋亡 [14] 、雌激素信号转导 [15] 、胰岛素抵抗 [16] 、组织发育 [1] 的文献中也用到ChIP。 目前，最常见的有以下两种 ChIP 实验技术： nChIP：用来研究 DNA 及高结合力蛋白，采用微球菌核酸酶（micrococcal nuclease）消化染色质，然后进行片段富集及后续分析，适用于组蛋白及其异构体，例如 [17