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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
2-Benzyl-1,2,3,4-tetrahydro-isoquinoline-3-carboxylic acid
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
54329-48-5
- 规格:
10mg///100mg///200mg
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文献和实验Communications【4-5】和 Cells【6】发表的三篇文章,整理了人多能干细胞(hPSC)来源的胰岛样类器官培养方案。该培养过程遵循发育原理,由 hPSC 依次分化为定型内胚层 (DE)、胰腺祖细胞 (PP)、内分泌前体 (EP), 最终诱导发育为人胰岛样类器官(human Islet-like Organoid, hILO)。 图 1. hPSC 来源细胞诱导成为胰岛样类器官的培养方案示意图【7】 细胞来源 hPSC 培养试剂配方 包被液 hPSC培养基 Day1 Day
Two-hybrid analysis of genetic
strain will contain a different bait plasmid. Prey strains: S.cerevisiae strain EGY48 (MATa ura3 his3 leu2::3LexAop-LEU2 trp1 LYS2)transformed with TRP1 prey plasmids,such as derivatives of pJG4-5 that produce different activation domain-tagged proteins
Preparation of probes for in situ hybridization
. Labeling is then conducted for 24-48 h in the dark, at room temperature, with occasional vortexing. NB: Any trace of free amine, such as Tris base, should be removed from the nucleic acid, as it will also react with the fluorophore.
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