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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 英文名:
pSH47-HIS3
- 保质期:
3个月~12个月
- 供应商:
上海禾午生物有限公司
- 保存条件:
负20℃&负80℃
- 规格:
干粉&甘油菌
| 名称 | pSH47-HIS3 |
| 别称 | - |
| 质粒宿主 | 酵母菌 |
| 质粒用途 | 基因编辑 |
| 片段类型 | CRISPR |
| 片段物种 | 空载体 |
| 原核抗性 | Amp |
| 筛选标记 | URA3,HIS3 |
| 启动子 | GAL1 |
| 复制子 | pUC |
| 感受态 | DH5a |
| 温度 | 37度 |
| 正向引物 | M13F |




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文献和实验Two-hybrid analysis of genetic
bait plasmid and a URA3 lacZ reporter plasmid like pSH18-34.The strain expressing the activation domain-tagged protein (prey strain)is EGY48 (MATa ura3 his3 leu2::3LexAop-LEU2 trp1 LYS2)transformed with the TRP1 prey plasmid.Patches of these two strains
Performing a hunt by interaction mating
-lacZ reporter usually resides on a multicopy (2µm) plasmid bearing a URA3 marker, such as pSH18-34. Again, various lacZ reporters with different numbers of upstream LexA operators exist. Strains with the entire lacZ plasmid integrated into the ura
Interaction Trap/Two‐Hybrid System to Identify Interacting Proteins
of interest Plasmids (see Table 4.4.1 ): e.g., pEG202 (Fig. ), pSH18‐34 (Fig. ), pSH17‐4, pRFHM1 Yeast strain: e.g., EGY48 (ura3 trp1 his3 3LexA ‐operator‐LEU2 ; see Table
技术资料








