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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
NHS-PEG-NHS (10 kDa)
- 库存:
999
- 供应商:
智溯科ZSK
- 规格:
10mg///100mg///200mg
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文献和实验Revised amino-allyl labeling protocol
as a dry pellet. Each tube is sufficient to label 10 reactions under normal conditions. Dissolve dry pellet in 20 m L DMSO. Aliquot 2 m L into 10 single use tubes that are then dried in vacuo and store desiccated at 4C. NHS-ester conjugated Cy dye
Protocol for Reverse Transcription and Amino-allyl Coupling of RNA
in pH. Monofunctional NHS-ester Cy3 (PA23001) and Cy5 dye (PA25001, Amersham) is supplied as a dry pellet. Each tube is sufficient to label 10 reactions under normal conditions. Dissolve dry pellet in 20 mL DMSO. Aliquot 2 mL into 10 single
and resuspend the washed microspheres in 80 μL 100 mM Monobasic Sodium Phosphate, pH 6.2 by vortex and sonication for approximately 20 seconds.7.Add 10 μL of 50 mg/mL Sulfo-NHS (diluted in dH20) to the microspheres and mix gently by vortex.8.Add 10 μL of 50 mg
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