阿狄科®粪便色氨酸检测试剂盒（酶联免疫法）

【产品名称】
通用名称：阿狄科®粪便色氨酸检测试剂盒（酶联免疫法）
英文名称：IDK® Tryptophane (Stool)
【包装规格】
规格：96人份（复孔检测样本：40个）/盒
测试次数：96（40份样本一式两份）
【预期用途】
用于定量测定所有年龄和性别患者粪便中游离 L-色氨酸的浓度。仅供科研使用。

【背景知识】
色氨酸是一种必需氨基酸，通过其各种主要代谢途径在微生物组-肠-脑轴中发挥重要作用[1]。这些包括血清素 (5-HT) 通路、犬尿氨酸通路、吲哚介导的芳烃受体 (AhR) 信号通路、mTOR（雷帕霉素的哺乳动物靶点）信号通路和蛋白质生物合成。
在人体中，摄入的色氨酸最多只有 1% 转化为血清素，而大部分（高达 90%）通过犬尿氨酸途径被吲哚胺-2,3-双加氧酶 (ID01) 转化为下游终产物烟酰胺腺嘌呤二核苷酸(NAD)、犬尿酸[2]和约5%的色氨酸代谢为吲哚，剩余的色氨酸用于蛋白质生物合成。
与肠易激综合征有关，色氨酸水平低可追溯到肠道菌群失衡（生态失调），并可能产生以下影响：

1. 肠黏膜中血清素（5-羟色胺，5-HT）水平降低，导致胃肠道运动受限和过敏反应增加[3,4,5]。
2. 减轻疼痛的色氨酸代谢物犬尿酸的产生减少，导致感觉知觉、胃肠道超敏反应和疼痛[6,7]增加。 在肠易激综合征患者的肠黏膜中发现犬尿酸含量降低[8]。
3. 肠道中 AhR 配体水平的降低，包括 indole-3-propionic 酸和 indole-3-lactate，导致 AhR 介导的信号传导减少和抗炎细胞因子的释放减少[9-13]。
4. mTOR 活性降低，导致黏膜界面蛋白的生物合成减少。这会导致黏膜通透性增加，从而使细菌能够穿透肠壁并导致肠漏综合征[14,15,16]。

参考文献：
1. Grifka-Walk HM, Jenkins BR, Kominsky DJ.Amino Acid Trp:TheFar Out lmpacts of Host and Commensal Tryptophan Metabolism.Front lmmunol.2021,12(June):1-14.doi:10.3389/fimmu.2021.653208
2. Li M,Liu S, Wang M, Hu H, Yin J, Liu C, Huang Y. Gut Microbiota Dysbiosis Associated with Bile Acid Metabolism in Neonatal Cholestasis Disease. Sci. Rep.2020,10,7686.
3. Coates MD, et al. Molecular defects in mucosal serotonin content and decreased serotonin reuptake transporter in ulcerative colitis and irritable bowel syndrome.Gastroenterology, 2004,126,1657-1664
4. Yazar A, Buyukafpar K, Polat G, et al.The urinary 5-hydroxyindole acetic acid and plasmanitric oxide levels in irritable bowel syndrome: a preliminary study. ScottMed J.2005,50,27-29
5. Dunlop SP,Coleman NS,Blackshaw E, etal. Abnormalities of 5-hydroxytryptamine metabolism in irritable bowel syndrome.Clin GastroenterolHepatol.2005,3(4): 349-357.doi:10.1016/s1542-3565(04)00726-8
6. Kennedy PJ, Cryan JF, Dinan TG, Clarke G.Kynurenine pathway metabolism and the microbiota-gut-brain axis. Neuropharmacology. 2017,112,399-412.
7. Wang J, Simonavicius N, Wu X, Swaminath G, Reagan J, Tian H, Ling L.Kynurenic acid as a ligand for orphan Gprotein-coupled receptor GPR35.J.Biol.Chem.2006,281,22021-22028
8. Keszthelyi D, Troost FJ,Jonkers DM, KruimelJW, Leue C,Masclee AAM.Decreased levels of kynurenic acid in the intestinal mucosa of lBS patients: Relation toserotonin and psychological state.J.Psychosom.Res.2013,74,501-504
9. Zhao ZH, Xin FZ, Xue Y,HuZ, Han Y, Ma F,Zhou D, Liu XL, CuiA, Liu Z, Liu Y,Gao J, Pan Q,Li Y,Fan.G.Indole-3-propionic acid inhibits gut dysbiosis and endotoxinleakage to attenuate steatohepatitis in rats.Exp Mol Med.2019,Sep 10;51(9):1-14.doi: 10.1038/s12276-019-0304-5
10.Zou J, Shen Y, Chen M, Zhang Z, Xiao S, Liu C, Wan Y,Yang L, Jiang S, Shang E,Qian D, Duan J. Lizhong. Decoction ameliorates ulcerative colitis in mice viamodulating gut microbiota and its metabolites.Appl Microbiol Biotechnol.2020Jul;104(13):5999-6012.doi:10.1007/s00253-020-10665-1
11.Roth W,Zadeh K, Vekariya R, Ge Y, Mohamadzadeh M.Tryptophan Metabolism and Gut-Brain Homeostasis. lnt J Mol Sci. 2021，Mar15;22(6):2973. doi:10.3390/ijms22062973
12.Scott SA, Fu J, Chang PV.Microbial tryptophan metabolites regulate gut barrier function via the aryl hydrocarbon receptor. Proc Natl Acad Sci U SA. 2020,Aug11;117(32):19376-19387.doi: 10.1073/pnas.2000047117
13. van Thiel lIAM，Botschuijver s, de Jonge WJ,Seppen J. Painful interactions:Microbial compounds and visceral pain.Biochim.Biophys. Acta Mol.Basis Dis.2020,1866,165534.
14.Liang H. DietaryL-Tryptophan Supplementation Enhances the Intestinal Mucosal Barrier Function in Weaned Piglets: lmplication of Tryptophan-MetabolizingMicrobiota.lnt.J.Mol.Sci.2019, 20,20
15.Peterson LW,Artis D. Intestinal epithelial cells: Regulators of barrier function and immune homeostasis. Nat.Rev.lmmunol.2014,14,141-153
16.Messori S,Trevisi P, Simongiovanni A, Priori D, Bosi P.Effect of susceptibility toenterotoxigenic Escherichia coli F4 and of dietary tryptophan on gut microbiotadiversity observed in healthy young pigs.Vet.Microbiol.2013,162,173-179