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- 详细信息
- 文献和实验
- 技术资料
- 库存:
170
- 英文名:
pCDNA3.1-HA-Gpc3(mouse)
- 保质期:
2年
- 供应商:
上海烜雅生物科技有限公司
- 保存条件:
2-8℃
- 规格:
少量干粉质粒/大提液体质粒
| 规格: | 少量干粉质粒 | 产品价格: | ¥380.0 |
|---|---|---|---|
| 规格: | 大提液体质粒 | 产品价格: | ¥580.0 |
别称:NM_016697.3;OCI-5
基因长:1740bp
质粒宿主:哺乳细胞
质粒用途:蛋白表达
片段类型:ORF
片段物种:小鼠
原核抗性:Amp
筛选标记:Neo/G418
荧光标记:
启动子:CMV
复制子:pUC
感受态:DH5a
温度:37度
正向引物:CMV-F:CGCAAATGGGCGGTAGGCGTG
反向引物:BGH:TAGAAGGCACAGTCGAGG
注意事项:本产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药。
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文献和实验Blastocyst embryo transfer into pseudopregnant recipient female mouse(图)
and exhibit bad planes of anesthesia when heavy. This is also a very inexpensive mouse to use. As an alternate, another strain I have used with considerable success is B6D2F1. These mice are hardy and display hybrid vigor. The Equipment For surgery
PROTOCOL General anesthesia inject i.p. 250µl/mouse of the ANESTHETIC mixture for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet) Preparation
Vasectomized males are needed to engender pseudopregnancy in mature female mice. Such pseudopregnant females act as hormonally and physiologically competent recipients for microinjected fertilized one-cell eggs (see Chapter 20 ). Vasectomized
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