- ￥765
- Sigma-Aldrich
- 进口
- S1885-500MG
- 2025年08月06日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8°C
- 保质期：
根据瓶身LOT号查询
- 英文名：
Suberic acid bis(N-hydroxysuccinimide ester)
- 库存：
有现货
- 供应商：
浙江羽翔生物科技有限公司
- CAS号：
68528-80-3
- 规格：
500MG
属性
质量水平
200
方案
≥95%
表单
powder
反应适用性
reagent type: cross-linking reagent
溶解性
chloroform: 50 mg/mL
DMF: soluble
aqueous buffer: slightly soluble (may require 5-10% organic solvent in coupling mixture.)
官能团
imide
储存温度
2-8°C
SMILES字符串
O=C(CCCCCCC(=O)ON1C(=O)CCC1=O)ON2C(=O)CCC2=O
InChI
1S/C16H20N2O8/c19-11-7-8-12(20)17(11)25-15(23)5-3-1-2-4-6-16(24)26-18-13(21)9-10-14(18)22/h1-10H2
InChI key
ZWIBGKZDAWNIFC-UHFFFAOYSA-N
一般描述
应用
- 对细胞裂解缓冲液进行补充，用于免疫沉淀
- 将寡核苷酸标准品与明胶缀合
- 交联ASC（凋亡相关斑点样蛋白，含胱天蛋白酶募集结构域）寡聚体
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文献和实验EpCAM homo-oligomerization is not the basis for its role in cell-cell adhesion.
Cell-surface tumor marker EpCAM plays a key role in proliferation, differentiation and adhesion processes in stem and epithelial cells. It is established as a cell-cell adhesion molecule, forming intercellular interactions through homophilic association. However, the mechanism by which such interactions arise has not yet been fully elucidated. Here, we first show that EpCAM monomers do not associate into oligomers that would resemble an inter-cellular homo-oligomer, capable of mediating cell-cell adhesion, by using SAXS, XL-MS and bead aggregation assays. Second, we also show that EpCAM forms stable dimers on the surface of a cell with pre-formed cell-cell contacts using FLIM-FRET; however, no inter-cellular homo-oligomers were detectable. Thus, our study provides clear evidence that EpCAM indeed does not function as a homophilic cell adhesion molecule and therefore calls for a significant revision of its role in both normal and cancerous tissues. In the light of this, we strongly support the previously suggested name Epithelial Cell Activating Molecule instead of the Epithelial Cell Adhesion Molecule.
【摘要】目的 构建和表达抗CD3/抗Pgp微型双功能抗体，并测定该微型双功能抗体的生物学活性。方法 采用PCR和overlap PCR方法构建抗CD3/抗CD20微型双功能抗体，并用双脱氧终止法测定DNA序列；采用亲和层析法纯化该产物，并用Western blot和分子排阻层析鉴定纯化产物；采用免疫荧光法、放射免疫分析法和玫瑰花环试验鉴定纯化产物与靶细胞的结合活性。结果 DNA序列测定结果表明：抗CD3/抗Pgp微型双功能抗体已构建成功，表达可溶性产物的产量达2mg/l以上，纯化产物中二
值比率（ 495/280 或 550/280 ），选取适当组分合并。 生物素标记抗体 抗体标记中生物素的引入使整个过程灵敏度大大提高！这种方法可使抗体或其它蛋白质的ε-氨基与酰化的生物素共价结合，生物素化的分子可以通过酶标记的抗生物素蛋白或荧光染料-链霉抗生物素蛋白复合物来检测。小分子水溶性生物素对链霉亲和素的高亲和力是该方法的设计基础。 操作步骤 1.用无水 DMSO 配制 10mg/ml 生物素 N- 羟基琥珀酰亚胺酯溶液。 2.硼酸盐缓冲液（0.1mol/L, pH8.8
In Situ Hybridization Using Digoxigenin Labeled Probes
Volume 1) Mix DNA (100-500 ng) + ddH2O and boil 5 minutes. 2) Cool immediately on ice (only critical for small probes, cosmids and P1's seem to do fine at room temp.) 3) Add remaining reagents 4) Incubate at room temp. for several hours to overnight
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