- ￥815
- Sigma-Aldrich
- 进口
- T1378-500G
- 2025年08月06日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温
- 保质期：
根据瓶身LOT号查询
- 英文名：
Sigma 7-9®
- 库存：
有现货
- 供应商：
浙江羽翔生物科技有限公司
- CAS号：
77-86-1
- 规格：
500G
属性
Product Name
Sigma 7-9®®, ≥99% (titration), powder or crystals
描述
aminopeptidase substrate
质量水平
200
方案
≥99% (titration)
表单
powder or crystals
储存条件
dry at room temperature
技术
ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
杂质
≤0.5% water (Karl Fischer)
颜色
white to off-white
pH值（酸碱度）
10.5-12
有效pH范围
7-9
pKa (25 °C)
8.1
沸点
219-220 °C/10 mmHg (lit.)
mp
167-172 °C (lit.)
溶解性
water: 0.667 g/mL, clear, colorless to faintly yellow
吸附
≤0.4 at 290 at 40%
应用
clinical research
diagnostic assay manufacturing
life science and biopharma
sample preparation
SMILES字符串
NC(CO)(CO)CO
InChI
1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key
LENZDBCJOHFCAS-UHFFFAOYSA-N
一般描述
此外，Tris 有助于增强细胞膜的渗透性。这种化合物在其用于制备与生物体液相容的缓冲液，作为标准 pH 溶液，促进乳酸脱氢酶测定、原位杂交和细胞蛋白质提取等实验方案的实验室中不可或缺。
应用
- 作为样品制备的缓冲液，用于涉及酶联免疫吸附测定(ELISA)的研究
- 作为样品制备缓冲液，用于免疫组织化学和免疫荧光相关研究
特点和优势
- 适用于生物和生化研究
- 在 pH 7-9 (25 °C)范围内有效缓冲，pKa 为 8.1 (25 °C)
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Intensity-dependent gamma electrical stimulation regulates microglial activation, reduces beta-amyloid load, and facilitates memory in a mouse model of Alzheimer's disease.
Gamma sensory stimulation may reduce AD-specific pathology. Yet, the efficacy of alternating electrical current stimulation in animal models of AD is unknown, and prior research has not addressed intensity-dependent effects. The intensity-dependent effect of gamma electrical stimulation (GES) with a sinusoidal alternating current at 40 Hz on Aβ clearance and microglia modulation were assessed in 5xFAD mouse hippocampus and cortex, as well as the behavioral performance of the animals with the Morris Water Maze. One hour of epidural GES delivered over a month significantly (1) reduced Aβ load in the AD brain, (2) increased microglia cell counts, decreased cell body size, increased length of cellular processes of the Iba1 + cells, and (3) improved behavioral performance (learning & memory). All these effects were most pronounced when a higher stimulation current was applied. The efficacy of GES on the reduction of AD pathology and the intensity-dependent feature provide guidance for the development of this promising therapeutic approach.
5%6%7.5%`10%12%15%water6.86.335.744.43.82.5450% glycerol0.10.110.110.120.20.31.5 M Tris, pH 8.833333310% SDS0.120.120.120.120.120.12Acrylamide22.4344.86TEMED (µl)55555510% persulfate (µl)353535353535Sample Buffers:Dehydrating Solution 30% Methanol
)。 2.3 试剂 1. 产品和液体贮存液 ( 1 ) 葡聚糖 T-500 和聚乙二醇（PEG ) 3350 分别购自 Amersham 和 Union Carbide。根据分子质量分别配制 20% 和 40% 的葡聚糖 T-500 和 PEG 3350 的贮存液（见注释 1)。 ( 2 ) 0.2 mol/L 的二水 EDTA 钠盐（的 EDTA - Na2) 贮存液的配置：500 ml 的水 ( 终体积）溶解 37.2 g 的 EDTA -Na2。在粉末中用 Trizma 碱（Sigma
Tween-20 Amresco 0777 Bromphenol Blue Amresco 0449 DTT Amresco 0281 Trizma base Sigma T1503 Protease inhibitor cocktail Roche 04693116001 Non-fat milk Erie yili
技术资料暂无技术资料 索取技术资料
