- ￥817
- Sigma-Aldrich
- 进口
- 81253-250G
- 2025年08月06日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温
- 保质期：
根据瓶身LOT号查询
- 英文名：
Poly(ethylene glycol)
- 库存：
有现货
- 供应商：
浙江羽翔生物科技有限公司
- CAS号：
25322-68-3
- 规格：
250G
属性
等级
Molecular Biology
质量水平
100
200
产品线
BioUltra
表单
solid
分子量
Mr 5000-7000
杂质
DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
pH值（酸碱度）
5.5-7.0 (25 °C, 50 mg/mL in H2O)
溶解性
H2O: 50 mg/mL at 20 °C, clear, colorless
痕量阴离子
chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg
痕量阳离子
Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤200 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤200 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg
λ
50 mg/mL in H2O
紫外吸收
λ: 260 nm Amax: 0.15
λ: 280 nm Amax: 0.10
适用性
suitable for (molecular biology applications)
SMILES字符串
C(CO)O
InChI
1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
InChI key
LYCAIKOWRPUZTN-UHFFFAOYSA-N
一般描述
应用
其他说明
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文献和实验Structural Insights into Stimulation of Ash1L's H3K36 Methyltransferase Activity through Mrg15 Binding.
The evolutionarily conserved Trithorax group protein Ash1 is a SET domain histone methyltransferase that mono- and dimethylates lysine 36 of histone H3 (H3K36). Ash1 forms a complex with Mrg15 and Nurf55, and the binding of Mrg15 greatly stimulates the catalytic activity of Ash1, yet the underlying molecular mechanisms remain unknown. Here we report the crystal structure of the tandem Mrg15-interacting and SET domains of human Ash1L in complex with Mrg15. Ash1L interacts with Mrg15 principally via a segment located N-terminal to the catalytic SET domain. Surprisingly, an autoinhibitory loop in the post-SET region of Ash1L is destabilized on Mrg15 binding despite no direct contact. Dynamics of the autoinhibitory loop can be attributed to subtle structural changes of the S-adenosylmethionine (SAM) binding pocket induced by Mrg15 binding, implicating a mechanism of conformational coupling between SAM and substrate binding sites. The findings broaden the understanding of regulation of H3K36 methyltransferases.
导致错误引发（False priming）。降低引物与模板相似性的一种方法是，引物中四种碱基的分布最好是随机的，不要有聚嘌呤或聚嘧啶的存在。尤其 3' 端不应超过 3 个连续的 G 或 C，因这样会使引物在 GC 富集序列区错误引发。 7、引物自身及引物之间不应存在互补序列 引物自身不应存在互补序列，否则引物自身会折叠成发夹结构（Hairpin）使引物本身复性。这种二级结构会因空间位阻而影响引物与模板的复性结合。引物自身不能有连续 4 个碱基的互补。两引物之间也不应具有互补性，尤其应避免
B1014 ADP,2Na,2H2 O (腺苷-5'-二磷酸腺苷) 100mg/250mg/1g 60/150/500 Sigma分装 B1017 Agar,Bacterological (琼脂粉-细菌培养级) 100g 100 日本分装 B
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of aggregation plate Materials: Dissecting microscope; Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3); 1 ml syringe with 26 G 1/2 needle of KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);
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