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- 文献和实验
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- 保存条件:
2-8°C
- 保质期:
6个月
- 库存:
100
- 供应商:
百欧泰
- 规格:
5ml/20ml
| 规格: | 5ml | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 20ml | 产品价格: | ¥2500.0 |
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文献和实验Preparation of Fluorescent DNA Probe from mRNA: Yeast
DTT (0.1 M) 3.0 7.5 10 mM 50X dNTPs 0.6 1.25 500 uM; 200 uM dTTP Cy3- or Cy5 dUTP 3.0
Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins
brighter than the wildtype GFPs (3 , 4 ). There are now blue and yellow fluorescent proteins and other colors may soon be available. The size of green fluorescent protein, about 28 kD (5 ,6 ), creates a large fluorescent tag in comparison to the 500
Non-fluorescent protein/RNA double-labeling
Non-fluorescent protein/RNA double-labeling (a version of this is described in: Manoukian, A. and Krause, H.M.(1992) Genes Dev ., 6: 1740-1751.) 1. Collect embryos, dechorionate and fix in a (1:1) mixture of heptane:fix. Fix
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