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Human R-Spondin 1 Recombinant

Protein
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  • ¥18888
  • GIBCO已认证
  • 120-38-500
  • 进口
  • 2026年02月11日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      充足

    • 英文名

      Human R-Spondin 1 Recombinant Protein

    • 规格

      500μg

    Human R-Spondin 1 Recombinant Protein(500 µg)

    产品细节图片1

    产品细节图片2

    产品细节图片3

    Product Details

    Size

    500 µg

    Species

    Human

    Published Species

    Dog, Pig, Bovine, Mouse, Human, Xenopus

    Expression system

    CHO cells

    Amino acid sequence

    SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP

    PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM

    ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT   VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA

    Molecular weight

    26.7 kDa

    Class

    Recombinant

    Type

    Protein

    Purity

    95% by SDS-PAGE gel and HPLC analyses.

    Endotoxin concentration

    <1 EU/µg

    Activity

    R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The expected ED50 is 1.0-3.0 ug/ml.

    Conjugate

    Unconjugated

    Form

    Lyophilized

    Purification

    purified

    Contains

    no preservative

    Storage conditions

    -20°C

     

    Applications

    Tested Dilution

    Publications

    Immunohistochemistry (IHC)

    -

    1 Publication

    Functional Assay (Functional)

    Assay-dependent

    -

    In vitro Assay (IV)

    -

    59 Publications

    Miscellaneous PubMed (Misc)

    -

    18 Publications

     

     

    Product Specific Information

    120-38-1MG will be provided as 2 x 500 µg (120-38-500UG).

    Recombinant Human R-Spondin-1 is a 26.7 kDa protein consisting of 243 amino acid residues. Due to glycosylation, R- Spondin-1 migrates at an apparent molecular weight of approximately 40.0 kDa by SDS-PAGE analysis under reducing conditions.

    This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot- specific Certificate of Analysis

    产品细节图片4

    Human R-Spondin 1 Protein (120-38-500UG) in Functional

    Bioassay analysis of Human R-Spondin 1 Recombinant Protein, PeproTech® (Product # 120-38-1MG).

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    图标文献和实验
    该产品被引用文献

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    [2] Molinuevo R, Menendez J, Cadle K, et al. Physiological DNA damage promotes functional endoreplication of mammary gland alveolar cells during lactation. Nat Commun. 2024;15(1):3288. Published 2024 Apr 17. doi:10.1038/s41467-024-47668-9

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    [5] Buchanan KL, Rupprecht LE, Kaelberer MM, et al. The preference for sugar over sweetener depends on a gut sensor cell. Nat Neurosci. 2022;25(2):191-200. doi:10.1038/s41593-021-00982-7

    [6] Nakamura K, Reid BM, Chen A, et al. Functional analysis of the 1p34.3 risk locus implicates GNL2 in high-grade serous ovarian cancer. Am J Hum Genet. 2022;109(1):116-135. doi:10.1016/j.ajhg.2021.11.020

    [7] Wang M, Yu H, Zhang T, et al. In-Depth Comparison of Matrigel Dissolving Methods on Proteomic Profiling of Organoids. Mol Cell Proteomics. 2022;21(1):100181. doi:10.1016/j.mcpro.2021.100181

    [8] Karvas RM, Khan SA, Verma S, et al. Stem-cell-derived trophoblast organoids model human placental development and susceptibility to emerging pathogens. Cell Stem Cell. 2022;29(5):810-825.e8. doi:10.1016/j.stem.2022.04.004

    [9] Chaves-Pérez A, Santos-de-Frutos K, de la Rosa S, Herranz-Montoya I, Perna C, Djouder N. Transit-amplifying cells control R-spondins in the mouse crypt to modulate intestinal stem cell proliferation. J Exp Med. 2022;219(11):e20212405. doi:10.1084/jem.20212405

    [10] Koikawa K, Kibe S, Suizu F, et al. Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy. Cell. 2021;184(18):4753-4771.e27. doi:10.1016/j.cell.2021.07.020

    [11] Moon S, Park Y, Hyeon S, et al. Niche-specific MHC II and PD-L1 regulate CD4+CD8αα+ intraepithelial lymphocyte differentiation. J Exp Med. 2021;218(4):e20201665. doi:10.1084/jem.20201665

    [12] Tajan M, Hennequart M, Cheung EC, et al. Serine synthesis pathway inhibition cooperates with dietary serine and glycine limitation for cancer therapy. Nat Commun. 2021;12(1):366. Published 2021 Jan 14. doi:10.1038/s41467-020-20223-y

    [13] Jurásek M, Kumar J, Paclíková P, et al. Phosphorylation-induced changes in the PDZ domain of Dishevelled 3. Sci Rep. 2021;11(1):1484. Published 2021 Jan 15. doi:10.1038/s41598-020-79398-5

    [14] Jurásek M, Kumar J, Paclíková P, et al. Phosphorylation-induced changes in the PDZ domain of Dishevelled 3. Sci Rep. 2021;11(1):1484. Published 2021 Jan 15. doi:10.1038/s41598-020-79398-5

    [15] Della Chiara G, Gervasoni F, Fakiola M, et al. Epigenomic landscape of human colorectal cancer unveils an aberrant core of pan-cancer enhancers orchestrated by YAP/TAZ. Nat Commun. 2021;12(1):2340. Published 2021 Apr 20. doi:10.1038/s41467-021-22544-y

    [16] Cheung VC, Peng CY, Marinić M, et al. Pluripotent stem cell-derived endometrial stromal fibroblasts in a cyclic, hormone-responsive, coculture model of human decidua. Cell Rep. 2021;35(7):109138. doi:10.1016/j.celrep.2021.109138

    [17] Francés-Herrero E, Juárez-Barber E, Campo H, et al. Improved Models of Human Endometrial Organoids Based on Hydrogels from Decellularized Endometrium. J Pers Med. 2021;11(6):504. Published 2021 Jun 3. doi:10.3390/jpm11060504

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    [24] Son YS, Ki SJ, Thanavel R, et al. Maturation of human intestinal organoids in vitro facilitates colonization by commensal lactobacilli by reinforcing the mucus layer. FASEB J. 2020;34(8):9899-9910. doi:10.1096/fj.202000063R

    [25] Chang TH, Shanti RM, Liang Y, et al. LGR5+ epithelial tumor stem-like cells generate a 3D-organoid model for ameloblastoma. Cell Death Dis. 2020;11(5):338. Published 2020 May 7. doi:10.1038/s41419-020-2560-7

    [26] Meir M, Burkard N, Ungewiß H, et al. Neurotrophic factor GDNF regulates intestinal barrier function in inflammatory bowel disease. J Clin Invest. 2019;129(7):2824-2840. Published 2019 Jun 17. doi:10.1172/JCI120261

    [27] Volk JK, Nyström EEL, van der Post S, et al. The Nlrp6 inflammasome is not required for baseline colonic inner mucus layer formation or function. J Exp Med. 2019;216(11):2602-2618. doi:10.1084/jem.20190679

    [28] Wagner S, Vlachogiannis G, De Haven Brandon A, et al. Suppression of interferon gene expression overcomes resistance to MEK inhibition in KRAS-mutant colorectal cancer. Oncogene. 2019;38(10):1717-1733. doi:10.1038/s41388-018-0554-z

    [29] Katsumi T, Guicciardi ME, Azad A, Bronk SF, Krishnan A, Gores GJ. Activated cholangiocytes release macrophage-polarizing extracellular vesicles bearing the DAMP S100A11. Am J Physiol Cell Physiol. 2019;317(4):C788-C799. doi:10.1152/ajpcell.00250.2019

    [30] Feldman A, Mukha D, Maor II, et al. Blimp1+ cells generate functional mouse sebaceous gland organoids in vitro. Nat Commun. 2019;10(1):2348. Published 2019 May 28. doi:10.1038/s41467-019-10261-6

    [31] Segal JM, Kent D, Wesche DJ, et al. Single cell analysis of human foetal liver captures the transcriptional profile of hepatobiliary hybrid progenitors. Nat Commun. 2019;10(1):3350. Published 2019 Jul 26. doi:10.1038/s41467-019-11266-x

    [32] Liu M, Sun T, Li N, et al. BRG1 attenuates colonic inflammation and tumorigenesis through autophagy-dependent oxidative stress sequestration. Nat Commun. 2019;10(1):4614. Published 2019 Oct 10. doi:10.1038/s41467-019-12573-z

    [33] Seishima R, Leung C, Yada S, et al. Neonatal Wnt-dependent Lgr5 positive stem cells are essential for uterine gland development. Nat Commun. 2019;10(1):5378. Published 2019 Nov 26. doi:10.1038/s41467-019-13363-3

    [34] Sachs N, de Ligt J, Kopper O, et al. A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity. Cell. 2018;172(1-2):373-386.e10. doi:10.1016/j.cell.2017.11.010

    [35] Kinchen J, Chen HH, Parikh K, et al. Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease. Cell. 2018;175(2):372-386.e17. doi:10.1016/j.cell.2018.08.067

    [36] Sechet E, Telford E, Bonamy C, Sansonetti PJ, Sperandio B. Natural molecules induce and synergize to boost expression of the human antimicrobial peptide β-defensin-3. Proc Natl Acad Sci U S A. 2018;115(42):E9869-E9878. doi:10.1073/pnas.1805298115

    [37] Koren E, Yosefzon Y, Ankawa R, et al. ARTS mediates apoptosis and regeneration of the intestinal stem cell niche. Nat Commun. 2018;9(1):4582. Published 2018 Nov 2. doi:10.1038/s41467-018-06941-4

    [38] Haider S, Meinhardt G, Saleh L, et al. Self-Renewing Trophoblast Organoids Recapitulate the Developmental Program of the Early Human Placenta. Stem Cell Reports. 2018;11(2):537-551. doi:10.1016/j.stemcr.2018.07.004

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    [40] Shibata W, Kinoshita H, Hikiba Y, et al. Overexpression of HER2 in the pancreas promotes development of intraductal papillary mucinous neoplasms in mice. Sci Rep. 2018;8(1):6150. Published 2018 Apr 18. doi:10.1038/s41598-018-24375-2

    [41] Garnier D, Li R, Delbos F, et al. Expansion of human primary hepatocytes in vitro through their amplification as liver progenitors in a 3D organoid system. Sci Rep. 2018;8(1):8222. Published 2018 May 29. doi:10.1038/s41598-018-26584-1

    [42] Bayrer JR, Wang H, Nattiv R, et al. LRH-1 mitigates intestinal inflammatory disease by maintaining epithelial homeostasis and cell survival. Nat Commun. 2018;9(1):4055. Published 2018 Oct 10. doi:10.1038/s41467-018-06137-w

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    [46] Zhang N, Huang H, Tan B, et al. Leucine-rich repeat-containing G protein-coupled receptor 4 facilitates vesicular stomatitis virus infection by binding vesicular stomatitis virus glycoprotein. J Biol Chem. 2017;292(40):16527-16538. doi:10.1074/jbc.M117.802090

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    [49] Kwon OJ, Zhang L, Xin L. Stem Cell Antigen-1 Identifies a Distinct Androgen-Independent Murine Prostatic Luminal Cell Lineage with Bipotent Potential. Stem Cells. 2016;34(1):191-202. doi:10.1002/stem.2217

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    [55] Karthaus WR, Iaquinta PJ, Drost J, et al. Identification of multipotent luminal progenitor cells in human prostate organoid cultures. Cell. 2014;159(1):163-175. doi:10.1016/j.cell.2014.08.017

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    [75] Ekanger CT, Zhou F, Bohan D, et al. Human Organotypic Airway and Lung Organoid Cells of Bronchiolar and Alveolar Differentiation Are Permissive to Infection by Influenza and SARS-CoV-2 Respiratory Virus. Front Cell Infect Microbiol. 2022;12:841447. Published 2022 Mar 14. doi:10.3389/fcimb.2022.841447

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