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蛋白长度 |
A DNA sequence encoding the Chikungunya virus (CHIKV)(strain SL-CK1) E2/Envelope 2 (ADG95913.1) (Ser326-Gln666) was expressed with a polyhistidine tag at the C-terminus.
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表达系统 |
Baculovirus-Insect Cells
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种属 |
CHIKV
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氨基酸序列 |
Ser326-Gln666
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标签类型 |
C-His
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分子量 |
The recombinant Chikungunya virus (CHIKV)(strain SL-CK1) E2/Envelope 2 consists of 352 amino acids and predicts a molecular mass of 39.8 kDa.
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活性 |
Testing in progress
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内毒素 |
< 1.0 EU per μg protein as determined by the LAL method.
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基因名称 |
CHIKV
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通用名 |
CHIKV
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形式 |
Lyophilized
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纯度 |
> 90 % as determined by SDS-PAGE.
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储存条件 |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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储存溶液 |
Lyophilized from sterile 20 mM Tris, pH 7.5, 500 mM NaCl, 10 % gly. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
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别名 |
Chikungunya virus;CHIKV
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文献和实验RT-PCR for the Assessment of Genetically Heterogenous Populations of the Hepatitis C Virus
have predominantly focused on two viral genetic loci, Hypervariable Region 1 (HVR 1) and the Interferon Sensitivity Determining Region (ISDR) (5 ) (Fig. 1 ). HVR 1 encodes an approx 27 amino-acid sequence at the N-terminal of envelope glycoprotein 2 (E2
Strategies to Optimize Protein Expression in E. coli
Bao, W.J., Gao, Y.G., Chang, Y.G., Zhang, T.Y., Lin, X.J., Yan, X.Z., and Hu, H.Y. 2006. Highly efficient expression and purification system of small‐size protein domains in Escherichia coli for biochemical characterization. Protein Expr
中科院高福院士课题组 2019 年发表了哪些重要的研究成果？
Alphavirus Receptor MXRA8 Binding to Chikungunya Virus Envelope Protein（甲病毒受体 MXRA8 与基孔肯雅病毒包膜蛋白结合的分子基础）虫源性甲病毒，如基孔肯雅病毒 (CHIKV)，可在全世界范围内引起严重和使人衰弱的风湿病，造成严重的经济损失。之前的研究发现 MXRA8 是病毒进入细胞的受体。在这里，研究人员揭示了小鼠 MXRA8、人 MXRA8 与 CHIKV E 蛋白复合物的晶体结构，以及人 MXRA8 和 CHIKV 病毒样颗粒的冷冻
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