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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
上海酶研生物科技有限公司
- 英文名：
PM-m (Pericyte Medium-m)
- 规格：
500ml/T
|
货号 |
1231 |
|
产地 |
北美 |
|
缩写 |
PM-m |
|
规格 |
500ml |
|
用途 |
科研 |
|
储存 |
4度，-20度 |
|
运输 |
胶冰 |
Pericyte Medium-mouse (PM-m), when used with Pericyte Growth Supplement (PGS-m, Cat #1282) and 10mL FBS is a complete medium designed for optimal growth of normal mouse pericytes in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (2%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal mouse pericytes in vitro.
31.) Guo W, Zhang D, Wang X, Kong W, Zhang Q, Zhang Y, Liu W. (2013) "Actin cytoskeleton modulates ADMA-induced NF-kappaB nuclear translocation and ICAM-1 expression in endothelial cells."?Med Sci Monit. 17: BR242
32.) Tong X, Peng H, Liu D, Ji L, Niu C, Ren J, Pan B, Hu J, Zheng L, Huang Y. (2013) "High-density lipoprotein of patients with Type 2 Diabetes Mellitus upregulates cyclooxgenase-2 expression and prostacyclin I-2 release in endothelial cells: relationship with HDL-associated sphingosine-1-phosphate."?Cardiovasc Diabetol. 12: 27.
33.) Yue X, Xu L, Zhu M, Zhang R, Liu C. (2013) "Over-expression of Nerve Growth Factor-α in human cholangiocarcinoma QBC939 cells promote tumor progression."?PLoS One. 8: e62024.
34.) Crowe A, Jackaman C, Beddoes KM, Ricciardo B, Nelson DJ. (2013) "Rapid copper acquisition by developing murine mesothelioma: decreasing bioavailable copper slows tumor growth, normalizes vessels and promotes T cell infiltration." PLoS One. 8: e73684.
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文献和实验
31.) Guo W, Zhang D, Wang X, Kong W, Zhang Q, Zhang Y, Liu W. (2013) "Actin cytoskeleton modulates ADMA-induced NF-kappaB nuclear translocation and ICAM-1 expression in endothelial cells."?Med Sci Monit. 17: BR242
32.) Tong X, Peng H, Liu D, Ji L, Niu C, Ren J, Pan B, Hu J, Zheng L, Huang Y. (2013) "High-density lipoprotein of patients with Type 2 Diabetes Mellitus upregulates cyclooxgenase-2 expression and prostacyclin I-2 release in endothelial cells: relationship with HDL-associated sphingosine-1-phosphate."?Cardiovasc Diabetol. 12: 27.
33.) Yue X, Xu L, Zhu M, Zhang R, Liu C. (2013) "Over-expression of Nerve Growth Factor-α in human cholangiocarcinoma QBC939 cells promote tumor progression."?PLoS One. 8: e62024.
34.) Crowe A, Jackaman C, Beddoes KM, Ricciardo B, Nelson DJ. (2013) "Rapid copper acquisition by developing murine mesothelioma: decreasing bioavailable copper slows tumor growth, normalizes vessels and promotes T cell infiltration." PLoS One. 8: e73684.
胎牛血清是细胞培养常用的添加物， 目前享有盛誉的Life等国际品牌的最好胎牛血清价格飞涨，500ML从原来的3000-4000元涨到现在7000-8000元，给很多科研单位或机构造成了巨大的压力，不管是科研单位还是经销商都费劲心机储备高质量的国际进口血清。然而前期储备的较低价格的进口血清总有用完的一天， 因此国内相关单位非常关注寻求新型的细胞培养基。 但并不是所有的细胞培养需要最高质量的胎牛血清， 而且市场上有些培养基完全可以替代昂贵的Life等国际品牌血清。 笔者对哺乳细胞培养过程中培养
100+ 种不同细胞全面验证— 翌圣生物转染试剂：高效低毒的国产优选
权威案例验证 原代鸡胚成纤维细胞（DNA） 原代小鼠神经干细胞（mRNA） 原代胶质母细胞瘤细胞（siRNA） 02 RNAiBoost-siRNA/miRNA 专用转染试剂 精准沉默，攻克难转体系 针对 siRNA/miRNA 转染 “敲低效率低、原代细胞难转、毒性大” 的行业痛点，翌圣生物依托 递送平台，研发出 RNAiBoost 专用转染试剂，专为 RNA 干扰场景优化，是原代细胞、悬浮细胞等难转
。但体外寿命有限，通常只能传代几次就会衰老死亡。 图 1. 原代细胞分离和培养技术-以小鼠原代肝细胞为例[1]。 『细胞系』 定义： 原代细胞经首次传代成功后，在体外具有无限增殖能力 (永生化) 或有限寿命的细胞群。——最常用，一般从国家或专业细胞资源库 (如 ATCC) 购买成熟的细胞系。 特点： 遗传背景明确，生长均一性好，易于基因编辑和大规模培养。 既包括有限传代细胞系 (传代次数有限)，也包括连续/永生化细胞系 (可长期传代)： (1) 有限传代
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