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- C-1001-NG
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- 2025年08月01日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
上海酶研生物科技有限公司
- 英文名：
ECM-NG (Endothelial Cell Medium-NG)
- 规格：
500ml/T
|
货号 |
1001-NG |
|
产地 |
北美 |
|
缩写 |
ECM-NG |
|
规格 |
500ml |
|
用途 |
科研 |
|
储存 |
4度，-20度 |
|
运输 |
胶冰 |
Endothelial cell medium – no glutamine (ECM-NG), when used with Endothelial Cell Growth Supplement (ECGS) and FBS is a complete medium designed for optimal growth of normal endothelial cells?in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal endothelial cells?in vitro.
ECM-NG does not contain L-glutamine,?an essential amino acid that is unstable in solution at 37°C. ECM-NG should be supplemented with L-glutamine (L-Glu; cat #0813) or stable L-glutamine dipeptide, L-alanyl-l-glutamine (LALG, Cat. #0733). The recommended LALG concentration ranges from 2mM to 10mM but may vary with culture conditions.
1.) Zhou H, Ji X, Wu Y, Xuan J, Qi Z, Shen L, Lan L, Li Q, Yin Z, Li Z, Zhao Z. (2014) 'A Dual-Role of Gu-4 in Suppressing HMGB1 Secretion and Blocking HMGB1 Pro-Inflammatory Activity during Inflammation.'
2.) Zhao N, Watson N, Xu Z, Chen Y, Waterman J, Sankar J, Zhu D. (2014) 'In Vitro Biocompatibility and Endothelialization of Novel Magnesium-Rare Earth Alloys for Improved Stent Applications.'
3.) Yang J, Wang Q, Qiao C, Lin Z, Li X, Huang Y, Zhou T, Li Y, Shen B, Lv M, Feng J. (2014) 'Potent anti-angiogenesis and anti-tumor activity of a novel human anti-VEGF antibody, MIL60.'
4.) Xu Q, Xia P, Li X, Wang W, Liu Z, Gao X. (2014) 'Tetramethylpyrazine Ameliorates High Glucose-Induced Endothelial Dysfunction by Increasing Mitochondrial Biogenesis.'
5.) Xing S, Yang X, Li W, Bian F, Wu D, Chi J, Xu G, Zhang Y, Jin S. (2014) 'Salidroside Stimulates Mitochondrial Biogenesis and Protects against H 2 O 2-Induced Endothelial Dysfunction.'
6.) Voigt J, Christensen J, Shastri VP. (2014) 'Differential uptake of nanoparticles by endothelial cells through polyelectrolytes with affinity for caveolae.'
7.) Wang Q, Yang J, Tang K, Luo L, Wang L, Tian L, Jiang Y, Feng J, Li Y, Shen B, Ly M, Huang Y. (2014) 'Pharmacological characteristics and efficacy of a novel anti-angiogenic antibody FD006 in corneal neovascularization.'
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文献和实验
1.) Zhou H, Ji X, Wu Y, Xuan J, Qi Z, Shen L, Lan L, Li Q, Yin Z, Li Z, Zhao Z. (2014) 'A Dual-Role of Gu-4 in Suppressing HMGB1 Secretion and Blocking HMGB1 Pro-Inflammatory Activity during Inflammation.'
2.) Zhao N, Watson N, Xu Z, Chen Y, Waterman J, Sankar J, Zhu D. (2014) 'In Vitro Biocompatibility and Endothelialization of Novel Magnesium-Rare Earth Alloys for Improved Stent Applications.'
3.) Yang J, Wang Q, Qiao C, Lin Z, Li X, Huang Y, Zhou T, Li Y, Shen B, Lv M, Feng J. (2014) 'Potent anti-angiogenesis and anti-tumor activity of a novel human anti-VEGF antibody, MIL60.'
4.) Xu Q, Xia P, Li X, Wang W, Liu Z, Gao X. (2014) 'Tetramethylpyrazine Ameliorates High Glucose-Induced Endothelial Dysfunction by Increasing Mitochondrial Biogenesis.'
5.) Xing S, Yang X, Li W, Bian F, Wu D, Chi J, Xu G, Zhang Y, Jin S. (2014) 'Salidroside Stimulates Mitochondrial Biogenesis and Protects against H 2 O 2-Induced Endothelial Dysfunction.'
6.) Voigt J, Christensen J, Shastri VP. (2014) 'Differential uptake of nanoparticles by endothelial cells through polyelectrolytes with affinity for caveolae.'
7.) Wang Q, Yang J, Tang K, Luo L, Wang L, Tian L, Jiang Y, Feng J, Li Y, Shen B, Ly M, Huang Y. (2014) 'Pharmacological characteristics and efficacy of a novel anti-angiogenic antibody FD006 in corneal neovascularization.'
性，一般与激素或其它物质起相互协同或加成作用。另一种常见的添加物就是血清替代物，目前已有许多可完全或部分替代传统培养液中血清成分的商业产品，其稳定性较好，但批次间仍有差别，产品的成分也不很确定。2、细胞培养基配制 2.1 干粉培养基原倍液的配制 1）配制过滤除菌的细胞培养基 1）阅读培养基使用说明，确定需要添加何种添加剂（如NaHCO3、L-谷氨酰胺、丙酮酸钠、HEPES等）。 2）根据所需将培养基全部倒入一容器中，用少量注射用水（20℃～30℃）将袋内残留培养基洗下，并入容器。加注射用水至总体
相关专题 MEM细胞培养基 GIBCO的MEM粉末包装上面有写：含谷氨酰胺，非必需氨基酸，但是不含碳酸氢钠，没有注明加碳酸氢钠的量。我是用的10%的血清的培养基，是不是配制培养基的时候用900ML的双蒸水就好了呢?需要加碳酸氢钠多少?还需要补充一些什么成分呢? 仔细看看MEM粉末的外包装标签，上面会有加碳酸氢钠的要求。如果没有，大包装盒上一定有，1L*10的大包装盒上有加碳酸氢钠的要求，1L加2.2g碳酸氢钠。否则请你的
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