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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海酶研生物科技有限公司
- 英文名:
ACM-sf(Astrocyte Conditioned Medium-Serum Free),100ml
- 规格:
500ml/T
|
货号 |
1811-sf |
|
产地 |
美国 |
|
缩写 |
ACM-sf |
|
规格 |
100 ml |
|
用途 |
科研 |
|
储存 |
4度,-20度 |
|
运输 |
胶冰 |
Astrocytes are the most abundant cells in the central nervous system (CNS). Astrocytes provide a variety of biological functions, such as supporting endothelial cells involved in blood-brain barrier formation, providing nutrients to neurons, maintaining extracellular ion balance, and promoting repair in the CNS. Astrocyte conditioned medium-serum free (ACM-sf) has been widely used to support the growth of neurons and endothelial cells in culture. ACM has also been shown to protect neurons from corticosterone-induced damage.
ACM-sf is a liquid medium prepared from primary human astrocyte cultures by using astrocyte medium (Cat #1801) without serum conditioned for 48 hours. The medium is sterile filtered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.
41.) Wu M, Zhao D, Zhong W, Yan H, Wang X, Liang Z, Li Z. (2013) 'High-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes.'
42.) Shi L, Zhang S, Wu H, Zhang L, Dai X, Hu J, Xue J, Liu T, Liang Y, Wu G. (2013) 'MiR-200c Increases the Radiosensitivity of Non-Small-Cell Lung Cancer Cell Line A549 by Targeting VEGF-VEGFR2 Pathway.'
43.) Muniz-Feliciano L, Van GJ, Portillo JAC, Liew L, Liu B, Carlin CR, Carruthers VB, Matthews S, Subauste CS. (2013) 'Toxoplasma gondii-Induced Activation of EGFR Prevents Autophagy Protein-Mediated Killing of the Parasite.'
44.) Kong X, Zhang Y, Liu C, Guo W, Li X, Su X, Wan H, Sun Y, Lin, N. (2013) 'Anti-Angiogenic Effect of Triptolide in Rheumatoid Arthritis by Targeting Angiogenic Cascade.'
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文献和实验
41.) Wu M, Zhao D, Zhong W, Yan H, Wang X, Liang Z, Li Z. (2013) 'High-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes.'
42.) Shi L, Zhang S, Wu H, Zhang L, Dai X, Hu J, Xue J, Liu T, Liang Y, Wu G. (2013) 'MiR-200c Increases the Radiosensitivity of Non-Small-Cell Lung Cancer Cell Line A549 by Targeting VEGF-VEGFR2 Pathway.'
43.) Muniz-Feliciano L, Van GJ, Portillo JAC, Liew L, Liu B, Carlin CR, Carruthers VB, Matthews S, Subauste CS. (2013) 'Toxoplasma gondii-Induced Activation of EGFR Prevents Autophagy Protein-Mediated Killing of the Parasite.'
44.) Kong X, Zhang Y, Liu C, Guo W, Li X, Su X, Wan H, Sun Y, Lin, N. (2013) 'Anti-Angiogenic Effect of Triptolide in Rheumatoid Arthritis by Targeting Angiogenic Cascade.'
【求助】请教!我用无血清培养基养的胃癌原代细胞,为什么会出现这种小颗粒?
暮色英雄 我是用无血清NB培养基+EGF+bFGF+B27+p/s双抗的培养基培养的胃癌原代细胞,培养基已经用0.22μm过滤器过滤除菌,但是培养细胞之后出现很多小颗粒,不知道是什么东西,这照片是昨天照的,今天再观察的时候这些小颗粒已经长满了,我师姐帮我看了说是污染,然后我把细胞倒掉了,但是她也不知道这是什么污染。希望各位大侠能帮小弟看看这到底是不是污染,是什么东西?谢谢了 这是20x镜拍
上清液。 PBS重悬的外泌体初始液 12,000 g,2 min 离心 2-3 次至无明显沉淀,每次离心收集上清液,上清液再进行EPF柱纯化。 建议:细胞培养使用低血清或者无外泌体血清或者外泌体专用无血清培养基,培养时间控制在24 - 48 h 左右 ,收集上清液时培养基颜色正常,不要等到偏黄色时再收集上清液。 11、EPF柱是否可以多次使用? 不能重复使用。过量的样品会超出纯化柱的使用极限,会严重影响分离效果。 12、外泌体如何保存? 纯化后的外泌体可于 4℃ 保存不超过一周,-80℃ 条件下可长期
在细胞生物学领域,原代细胞培养作为解开生命奥秘的重要工具,要求实验者遵循一系列关键的注意事项以确保可靠的实验结果。 本文将深入探讨如何在原代细胞培养过程中应用这些关键要点: 原代培养要重点注意无菌操作,所用器械需提前高压灭菌; 鼠胚原代培养时,获取组织较少,建议在冷光源体视显微镜下操作; 原代细胞培养过程比较慢,培养时间最少需要一周的时间。若 2 天后可能会出现培养基变黄的现象,且无漂浮物,排除污染,属于正常现象。这是因为细胞在贴壁生长过程中释放了 CO2 和其他物质导致培养液 PH
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