支气管上皮细胞培养基

支气管上皮细胞培养基是为正常人类支气管上皮细胞体外培养设计的适于其生长的培养基。无血清培养基是无菌的、液体培养基，包含必需和非必需氨基、维生素、有机和无机化合物、激素、生长因子、微量矿物质。该培养基不含血清。该培养基含碳酸氢盐缓冲体系，在5%二氧化碳/95%空气培养箱中平衡时PH值为7.4。该培养基在数量上和质量上都保证理想的营养平衡状态，选择性促进体外正常人类支气管上皮细胞的生长。
成分
  支气管上皮细胞培养基包含500 ml基础培养基，5 ml支气管上皮细胞生长添加物，(BEpiCGS,目录编号3262)和5 ml青霉素/链霉素溶液(P/S,目录编号0503)

1. Bai, X.Y., Zhang, X.C., Yang, S.Q., An, S.J., Chen, Z.H., Su, J., Xie, Z., Gou, L.Y. & Wu, Y.L. (2016) Blockade of Hedgehog Signaling Synergistically Increases Sensitivity to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors in Non-Small-Cell Lung Cancer Cell Lines PLoS One. 11

Aberrant activation of the hedgehog (Hh) signaling pathway has been implicated in the epithelial-to-mesenchymal transition (EMT) and cancer stem-like cell (CSC) maintenance; both processes can result in tumor progression and treatment resistance in several types of human cancer. Hh cooperates with the epidermal growth factor receptor (EGFR) signaling pathway in embryogenesis. We found that the Hh signaling pathway was silenced in EGFRTKI-sensitive non-small-cell lung cancer (NSCLC) cells, while it was inappropriately activated in EGFR-TKI-resistant NSCLC cells, accompanied by EMT induction and ABCG2 overexpression. Upregulation of Hh signaling through extrinsic SHH exposure downregulated E-cadherin expression and elevated Snail and ABCG2 expression, resulting in gefitinib tolerance (P < 0.001) in EGFR-TKI-sensitive cells. Blockade of the Hh signaling pathway using the SMO antagonist SANT-1 restored E-cadherin expression and downregulate Snail and ABCG2 in EGFR-TKI-resistant cells. A combination of SANT-1 and gefitinib markedly inhibited tumorigenesis and proliferation in EGFR-TKI-resistant cells (P < 0.001). These findings indicate that hyperactivity of Hh signaling resulted in EGFR-TKI resistance, by EMT introduction and ABCG2 upregulation, and blockade of Hh signaling synergistically increased sensitivity to EGFR-TKIs in primary and secondary resistant NSCLC cells. E-cadherin expression may be a potential biomarker of the suitability of the combined application of an Hh inhibitor and EGFR-TKIs in EGFR-TKI-resistant NSCLCs. Less

2. Crowley, C., Klanrit, P., Butler, C.R., Varanou, A., Plat‚, M., Hynds, R.E., Chambers, R.C., Seifalian, A.M., Birchall, M.A. & Janes, S.M. (2016) Surface modification of a POSS-nanocomposite material to enhance cellular integration of a synthetic bioscaffold Biomaterials. 83

Polyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane (POSS-PCU) is a versatile nanocomposite biomaterial with growing applications as a bioscaffold for tissue engineering. Integration of synthetic implants with host tissue can be problematic but could be improved by topographical modifications. We describe optimization of POSS-PCU by dispersion of porogens (sodium bicarbonate (NaHCO3), sodium chloride (NaCl) and sucrose) onto the material surface, with the principle aim of increasing surface porosity, thus providing additional opportunities for improved cellular and vascular ingrowth. We assess the effect of the porogens on the material's mechanical strength, surface chemistry, wettability and cytocompatibilty. Surface porosity was characterized by scanning electron microscopy (SEM). There was no alteration in surface chemistry and wettability and only modest changes in mechanical properties were detected. The size of porogens correlated well with the porosity of the construct produced and larger porogens improved interconnectivity of spaces within constructs. Using primary human bronchial epithelial cells (HBECs) we demonstrate moderate in vitro cytocompatibility for all surface modifications; however, larger pores resulted in cellular aggregation. These cells were able to differentiate on POSS-PCU scaffolds. Implantation of the scaffold in vivo demonstrated that larger pore sizes favor cellular integration and vascular ingrowth. These experiments demonstrate that surface modification with large porogens can improve POSS-PCU nanocomposite scaffold integration and suggest the need to strike a balance between the non-porous surfaces required for epithelial coverage and the porous structure required for integration and vascularization of synthetic scaffolds in future construct design. Keywords: Biocompatible materials; Nanocomposites; Porosity; Re-epithelialization; Tissue engineering; Trachea. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved. Less