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- Sciencell
- B-R1811-sf
- 美国
- 2025年08月01日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
上海酶研生物科技有限公司
- 英文名：
RACM-sf（Rat Astrocyte Conditioned Medium-serum free）,100ml
- 规格：
100ml/T
|
货号 |
R1811-sf |
|
产地 |
美国 |
|
缩写 |
RACM-sf |
|
规格 |
100 ml |
|
用途 |
科研 |
|
储存 |
4度，-20度 |
|
运输 |
胶冰 |
Astrocytes are the most abundant cells in the central nervous system (CNS). Astrocytes provide a variety of biological functions, such as supporting endothelial cells involved in blood-brain barrier formation, providing nutrients to neurons, maintaining extracellular ion balance, and promoting repair in the CNS. Astrocyte conditioned medium-serum free (ACM-sf) has been widely used to support the growth of neurons and endothelial cells in culture. ACM has also been shown to protect neurons from corticosterone-induced damage .
Rat ACM-sf is a liquid medium prepared from primary rat astrocyte cultures by using astrocyte medium (Cat #1801) without serum and conditioned for 48 hours. The medium is sterile filtered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.
26.) Yang J, Wang Q, Qiao C, Lin Z, Li X, Huang Y, Zhou T, Li Y, Shen B, Lv M, Feng J. (2014) "Potent anti-angiogenesis and anti-tumor activity of a novel human anti-VEGF antibody, MIL60."?Cell molimmunol. 11: 285-93.
27.) Zhao N, Watson N, Xu Z, Chen Y, Waterman J, Sankar J, Zhu D. (2014) "In Vitro Biocompatibility and Endothelialization of Novel Magnesium-Rare Earth Alloys for Improved Stent Applications."?PloS one. 9: e98674.
28.) Zhou H, Ji X, Wu Y, Xuan J, Qi Z, Shen L, Lan L, Li Q, Yin Z, Li Z, Zhao Z. (2014) "A Dual-Role of Gu-4 in Suppressing HMGB1 Secretion and Blocking HMGB1 Pro-Inflammatory Activity during Inflammation."?PloS one. 9: e89634.
29.) Jandu SK, Webb AK, Pak A, Sevinc B, Nyhan D, Belkin AM, Flavahan NA, Berkowitz DE, Santhanam L. (2013) Nitric oxide regulates tissue transglutaminase localization and function in the vasculature.?Amino Acids. 44: 261-9.
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文献和实验
26.) Yang J, Wang Q, Qiao C, Lin Z, Li X, Huang Y, Zhou T, Li Y, Shen B, Lv M, Feng J. (2014) "Potent anti-angiogenesis and anti-tumor activity of a novel human anti-VEGF antibody, MIL60."?Cell molimmunol. 11: 285-93.
27.) Zhao N, Watson N, Xu Z, Chen Y, Waterman J, Sankar J, Zhu D. (2014) "In Vitro Biocompatibility and Endothelialization of Novel Magnesium-Rare Earth Alloys for Improved Stent Applications."?PloS one. 9: e98674.
28.) Zhou H, Ji X, Wu Y, Xuan J, Qi Z, Shen L, Lan L, Li Q, Yin Z, Li Z, Zhao Z. (2014) "A Dual-Role of Gu-4 in Suppressing HMGB1 Secretion and Blocking HMGB1 Pro-Inflammatory Activity during Inflammation."?PloS one. 9: e89634.
29.) Jandu SK, Webb AK, Pak A, Sevinc B, Nyhan D, Belkin AM, Flavahan NA, Berkowitz DE, Santhanam L. (2013) Nitric oxide regulates tissue transglutaminase localization and function in the vasculature.?Amino Acids. 44: 261-9.
机械性损伤导致细胞死亡等缺点。本实验采用视神经组织块培养的方法，对新生大鼠的视神经胶质细胞进行条件化原代培养，利用少突胶质细胞和星形胶质细胞生长条件的不同，采用条件培养基纯化2种细胞。少突胶质细胞和Ⅱ型星形胶质细胞是从一种普通双潜能的少突胶质细胞－2型星形胶质细胞祖细胞（oligodendrocyte-type-2astrocyte progenitor cell，O2A）发育而来。大鼠出生后约1周，少突胶质细胞逐渐成熟。因此，从新生大鼠取材可获得O2A祖细胞。体外研究发现甲状腺素、胰岛素、转铁
R&D Systems 适用于神经细胞培养的无血清培养基添加剂
r&d Systems如今提供两种新的无血清培养基添加剂，是专为神经细胞的培养而优化的。 N21-MaX Media Supplement: 适合无星形胶质细胞滋养层的神经元长期培养 无血清培养基添加剂，与神经细胞培养的基础培养基共同使用，为神经元的长期培养提供了一个营养丰富且完全限定的培养基，而无需星形胶质细胞滋养层。N21-MaX Media Supplement包含21种不同组分，以支持培养中神经元的长期生存。它经过验证，可支持e18大鼠海马神经元的生存和生长
上清液。 PBS重悬的外泌体初始液 12,000 g，2 min 离心 2-3 次至无明显沉淀，每次离心收集上清液，上清液再进行EPF柱纯化。 建议：细胞培养使用低血清或者无外泌体血清或者外泌体专用无血清培养基，培养时间控制在24 - 48 h 左右 ，收集上清液时培养基颜色正常，不要等到偏黄色时再收集上清液。 11、EPF柱是否可以多次使用？ 不能重复使用。过量的样品会超出纯化柱的使用极限，会严重影响分离效果。 12、外泌体如何保存？ 纯化后的外泌体可于 4℃ 保存不超过一周，-80℃ 条件下可长期
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