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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海酶研生物科技有限公司
- 英文名:
LCM(Leydig Cell Medium )
- 规格:
500ml/T
|
货号 |
4511 |
|
产地 |
美国 |
|
缩写 |
LCM |
|
规格 |
500ml |
|
用途 |
科研 |
|
储存 |
4度,-20度 |
|
运输 |
胶冰 |
Leydig Cell Medium (LCM) is a complete medium designed for optimal growth of normal Human Leydig Cells in vitro. It is a sterile, liquid medium containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is serum-free. It is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes growth of normal Human Leydig Cells in vitro.
Note: Due to bovine pituitary extract (BPE) in the growth supplement, the color may vary with different lots and formation of lipoproteins can result in precipitates. This does not affect the biological activity.
21.) Umar MI, Asmawi MZ, Sadikun A, Majid AMSA, Al-Suede FSR, Hassan LEA, Altaf R, Ahamed MBK. (2014) "Ethyl-p-methoxycinnamate isolated from kaempferiagalanga inhibits inflammation by suppressing interleukin-1, tumor necrosis factor-&aplha;, and angiogenesis by blocking endothelial functions."?Clinics. 69: 134-44.
22.) Voigt J, Christensen J, Shastri VP. (2014) "Differential uptake of nanoparticles by endothelial cells through polyelectrolytes with affinity for caveolae."?ProcNatlAcad Sci. 111: 2942-47.
23.) Wang Q, Yang J, Tang K, Luo L, Wang L, Tian L, Jiang Y, Feng J, Li Y, Shen B, Ly M, Huang Y. (2014) "Pharmacological characteristics and efficacy of a novel anti-angiogenic antibody FD006 in corneal neovascularization."?BMC biotechnol. 14: 17.
24.) Xing S, Yang X, Li W, Bian F, Wu D, Chi J, Xu G, Zhang Y, Jin S. (2014) "Salidroside Stimulates Mitochondrial Biogenesis and Protects against H 2 O 2-Induced Endothelial Dysfunction."?Oxid med cell longev. 2014: 904834.
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文献和实验
22.) Voigt J, Christensen J, Shastri VP. (2014) "Differential uptake of nanoparticles by endothelial cells through polyelectrolytes with affinity for caveolae."?ProcNatlAcad Sci. 111: 2942-47.
23.) Wang Q, Yang J, Tang K, Luo L, Wang L, Tian L, Jiang Y, Feng J, Li Y, Shen B, Ly M, Huang Y. (2014) "Pharmacological characteristics and efficacy of a novel anti-angiogenic antibody FD006 in corneal neovascularization."?BMC biotechnol. 14: 17.
24.) Xing S, Yang X, Li W, Bian F, Wu D, Chi J, Xu G, Zhang Y, Jin S. (2014) "Salidroside Stimulates Mitochondrial Biogenesis and Protects against H 2 O 2-Induced Endothelial Dysfunction."?Oxid med cell longev. 2014: 904834.
原代细胞分离和培养基本步骤1、器官和组织的选择尽可能的去除不必要的组织和血迹。2、原代细胞的分离(1)应用PriCells原代细胞分离试剂盒I、II、III。(2)根据组织来源不同,可选用不同的PriCells原代细胞分离试剂盒。3、原代细胞的培养:(1)PriCells原代细胞特制培养基(2)PriCells原代细胞特制添加物(3)优质胎牛血清4、细胞的培养和鉴定:PriCells原代细胞鉴定试剂盒5、原代细胞的传代、保存(1)传代:依椐细胞的生长状态,生长的细胞1:2或1:3进行传代
【求助】请教!我用无血清培养基养的胃癌原代细胞,为什么会出现这种小颗粒?
暮色英雄 我是用无血清NB培养基+EGF+bFGF+B27+p/s双抗的培养基培养的胃癌原代细胞,培养基已经用0.22μm过滤器过滤除菌,但是培养细胞之后出现很多小颗粒,不知道是什么东西,这照片是昨天照的,今天再观察的时候这些小颗粒已经长满了,我师姐帮我看了说是污染,然后我把细胞倒掉了,但是她也不知道这是什么污染。希望各位大侠能帮小弟看看这到底是不是污染,是什么东西?谢谢了 这是20x镜拍
在细胞生物学领域,原代细胞培养作为解开生命奥秘的重要工具,要求实验者遵循一系列关键的注意事项以确保可靠的实验结果。 本文将深入探讨如何在原代细胞培养过程中应用这些关键要点: 原代培养要重点注意无菌操作,所用器械需提前高压灭菌; 鼠胚原代培养时,获取组织较少,建议在冷光源体视显微镜下操作; 原代细胞培养过程比较慢,培养时间最少需要一周的时间。若 2 天后可能会出现培养基变黄的现象,且无漂浮物,排除污染,属于正常现象。这是因为细胞在贴壁生长过程中释放了 CO2 和其他物质导致培养液 PH
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