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- A-2611
- 美国
- 2025年08月01日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
上海酶研生物科技有限公司
- 英文名：
OKM (Oral Keratinocyte Medium)
- 规格：
500ml/T
|
货号 |
2611 |
|
产地 |
美国 |
|
缩写 |
OKM |
|
规格 |
500ml |
|
用途 |
科研 |
|
储存 |
4度，-20度 |
|
运输 |
胶冰 |
口腔角质细胞培养基是为正常人类口腔角化细胞体外培养设计的适于其生长的培养基。无血清培养基是无菌的、液体培养基，包含必需和非必需氨基、维生素、有机和无机化合物、激素、生长因子、微量矿物质。该培养基不含血清。该培养基含碳酸氢盐缓冲体系，在5%二氧化碳/95%空气培养箱中平衡时PH值为7.4。该培养基在数量上和质量上都保证理想的营养平衡状态，选择性促进体外正常人类神经元的生长。
口腔角化细胞培养基包含500 ml基础培养基，5 ml口腔角化细胞生长添加物，(OKGS,目录编号2652)和5 ml青霉素/链霉素溶液(P/S,目录编号0503)。注意：由于生长因子中的BPE，脂蛋白的形成可导致出现沉淀，出现多种颜色。
1.) Yen YC, Shiah SG, Chu HC, Hsu YM, Hsiao JR, Chang JY, Hugn WC, Liao CT, Cheng AJ, Lu YC, Chen YW. (2014) 'Reciprocal regulation of MicroRNA-99a and insulin-like growth factor I receptor signaling in oral squamous cell carcinoma cells.' Mol cancer.
2.) Yen YC, Shiah SG, Chu HC, Hsu YM, Hsiao JR, Chang JY, Hugn WC, Liao CT, Cheng AJ, Lu YC, Chen YW. (2014) "Reciprocal regulation of MicroRNA-99a and insulin-like growth factor I receptor signaling in oral squamous cell carcinoma cells." Mol cancer. 13: 6.
3.) Ji W, Lee C, Chen JY, Cheng Y, Yang S, Chen J, Chen H. (2013) "Areca Nut Extract Induces Pyknotic Necrosis in Serum-Starved Oral Cells via Increasing Reactive Oxygen Species and Inhibiting GSK3?: An Implication for Cytopathic Effects in Betel Quid Chewers." PLoS One. 8: e63295.
4.) Wallet MA, Calderon NL, Alonso TR, Choe CS, Catalfamo DL, Lalane CJ, Naiva KG, Panagakos F, Wallet SM. (2013) ?Triclosan alters antimicrobial and inflammatory responses of epithelial cells.? Oral dis. 19: 296-302.
5.) Chen IC, Chiang WF, Liu SY, Chen PF, Chiang HC. (2013) "Role of SIRT3 in the regulation of redox balance during oral carcinogenesis." Mol Cancer. 12: 68.
6.) Lee SS, Tsai CH, Yu CC, Chang YC. (2013) "Elevated Snail Expression Mediates Tumor Progression in Areca Quid Chewing-Associated Oral Squamous Cell Carcinoma via Reactive Oxygen Species." PLoS One. 8: e67985.
7.) Minakawa Y, Kasamatsu A, Koike H, Higo M, Nakashima D, Kouzu Y,Sakamoto Y, Ogawara K, Shiiba M, Tanzawa H,Uzawa K. (2013) 'Kinesin Family member 4A: A Potential Predictor for Progression of Human Oral Cancer.'
8.) Minakawa Y, Kasamatsu A, Koike H, Higo M, Nakashima D, Kouzu Y,Sakamoto Y, Ogawara K, Shiiba M, Tanzawa H,Uzawa K. (2013) "Kinesin Family member 4A: A Potential Predictor for Progression of Human Oral Cancer." PloS one. 8: e85951.
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文献和实验
1.) Yen YC, Shiah SG, Chu HC, Hsu YM, Hsiao JR, Chang JY, Hugn WC, Liao CT, Cheng AJ, Lu YC, Chen YW. (2014) 'Reciprocal regulation of MicroRNA-99a and insulin-like growth factor I receptor signaling in oral squamous cell carcinoma cells.' Mol cancer.
2.) Yen YC, Shiah SG, Chu HC, Hsu YM, Hsiao JR, Chang JY, Hugn WC, Liao CT, Cheng AJ, Lu YC, Chen YW. (2014) "Reciprocal regulation of MicroRNA-99a and insulin-like growth factor I receptor signaling in oral squamous cell carcinoma cells." Mol cancer. 13: 6.
3.) Ji W, Lee C, Chen JY, Cheng Y, Yang S, Chen J, Chen H. (2013) "Areca Nut Extract Induces Pyknotic Necrosis in Serum-Starved Oral Cells via Increasing Reactive Oxygen Species and Inhibiting GSK3?: An Implication for Cytopathic Effects in Betel Quid Chewers." PLoS One. 8: e63295.
4.) Wallet MA, Calderon NL, Alonso TR, Choe CS, Catalfamo DL, Lalane CJ, Naiva KG, Panagakos F, Wallet SM. (2013) ?Triclosan alters antimicrobial and inflammatory responses of epithelial cells.? Oral dis. 19: 296-302.
5.) Chen IC, Chiang WF, Liu SY, Chen PF, Chiang HC. (2013) "Role of SIRT3 in the regulation of redox balance during oral carcinogenesis." Mol Cancer. 12: 68.
6.) Lee SS, Tsai CH, Yu CC, Chang YC. (2013) "Elevated Snail Expression Mediates Tumor Progression in Areca Quid Chewing-Associated Oral Squamous Cell Carcinoma via Reactive Oxygen Species." PLoS One. 8: e67985.
7.) Minakawa Y, Kasamatsu A, Koike H, Higo M, Nakashima D, Kouzu Y,Sakamoto Y, Ogawara K, Shiiba M, Tanzawa H,Uzawa K. (2013) 'Kinesin Family member 4A: A Potential Predictor for Progression of Human Oral Cancer.'
8.) Minakawa Y, Kasamatsu A, Koike H, Higo M, Nakashima D, Kouzu Y,Sakamoto Y, Ogawara K, Shiiba M, Tanzawa H,Uzawa K. (2013) "Kinesin Family member 4A: A Potential Predictor for Progression of Human Oral Cancer." PloS one. 8: e85951.
人皮肤角质形成细胞的分离与原代无血清培养.pdf
。 5. 角质形成细胞使用5ml完全培养基轻柔的吹打成悬液，并使用血细胞计数器计算角质形成细胞浓度。原代细胞大约3×10 6 放入75cm 2 组织培养瓶中，并加入15ml完全培养基。 细胞培养 ：松弛的瓶盖，36°C ± 2°C，5% CO 2 的潮湿空气中。 6. 角质形成 细胞培养 液约每2～3天更换一次。 7. 由于原代角质形成细胞在供者之间
基无法满足的。新的3D培养基配方独特，能同时满足3D模型的增殖和分化。 CELLnTEC新的3D表皮角质形成细胞培养基是根据CELLnTEC的原代角质形成细胞特别优化的，与Millipore的Millicell inserts共同使用，能为3D体外皮肤模型创造出极佳的实验环境，代表了细胞培养性能的新水平，同时也非常便利和经济。 Millipore从一个高品质过滤产品和服务的公司逐步发展成为工具和服务的领先供应商，并成为生命科学用户精选的重要合作伙伴。我们通过研发投资，有机增长
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