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- 2025年08月01日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
pcDNA3.2-Cx43-HA
- 保质期：
3个月~12个月
- 供应商：
上海禾午生物有限公司
- 保存条件：
负20℃＆负80℃
- 规格：
干粉＆甘油菌
| 名称 | pcDNA3.2-Cx43-HA |
| 别称 | SC135844 |
| 基因长 | 1227bp |
| 质粒宿主 | 哺乳细胞 |
| 质粒用途 | 蛋白表达 |
| 片段类型 | ORF |
| 片段物种 | 人 |
| 原核抗性 | Amp |
| 筛选标记 | Neo/G418 |
| 启动子 | CMV |
| 复制子 | pUC |
| 感受态 | DB3.1 |
| 温度 | 37度 |
| 正向引物 | CMV-F2 |
| pCMV-3×FLAG-Arc(mouse)-Neo |
| pCMV-SCGB2A2(human)-3×HA-Neo |
| pCMV-T7-DHX8(human)-3×FLAG-Neo |
| pCMV-mCherry-CGAS(human)-3×HA-Neo |
| pcDNA3.2-Cx43-HA |
| pCMV-EGFP-IQGAP1(human)-3×Myc-Puro |
| pCMV-Usp8(mouse)-6×His-Neo |
| pCMV-attB1-Myc-CUL4A(human)-attB2-FLAG-Neo |
| pCMV-PTPRS(human)-Neo |
| pBiFC-CMV-PARP1(human)-VN155(I152L) |
| pCMV-Myh10(mouse)-3×FLAG-Neo |
| pCMV-FTO(human)-3×HA-Neo |
| pCMV-RELA(human)-3×HA-WPRE-Neo |
| pCMV-PRDX6(human)-3×FLAG-Neo |
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文献和实验Recombinant DNA Engineering, Or Cloning Genes In Plasmids
the plasmid DNA with very high fidelity. Plasmid DNA is physically independent of genomic DNA and this allows for their easy isolation from bacterial cultures. the pcDNA3.1+ plasmidVector plasmids used for cloning are short, circular, double-stranded DNA
Hi guys, I have a question for you. I made a construct which had been sequenced. It is in pcDNA3.1(+) and with HA-TAA to stop. The right size for endogenous protein is 31kD. But when I transfected into COS-7 and run western, the size
Detection of ERK1/2 Activities Using Affinity Reagents
-7 cells were transfected with pCDNA3-HA-ERK2 or pECE-HA-MAPK (HA-p38α) in six-well plates using the DEAE-dextran method (1 μg of DNA/well). Cells were activated with 50 ng/mL of EGF for 5 min (for HA-ERK2; lanes 1 and 2) or with 0.5 M sorbitol for 20 min
技术资料
