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- Sciencell
- B-M1811-57
- 美国
- 2025年08月01日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
上海酶研生物科技有限公司
- 英文名：
MACM-57（Mouse Astrocyte Conditioned Medium from C57BL/6）,100ml
- 规格：
500ml/T
|
货号 |
M1811-57 |
|
产地 |
美国 |
|
缩写 |
MACM-57 |
|
规格 |
100ml |
|
用途 |
科研 |
|
储存 |
4度 |
|
运输 |
胶冰 |
Astrocytes are the most abundant cells in the central nervous system (CNS). Astrocytes provide a variety of biological functions, such as supporting endothelial cells involved in blood-brain barrier formation, providing nutrients to neurons, maintaining extracellular ion balance, and promoting repair in the CNS. Astrocyte conditioned medium (ACM) has been widely used to support the growth of neurons and endothelial cells in culture. ACM has also been shown to protect neurons from corticosterone-induced damage [1].
Mouse ACM-57 is a liquid medium prepared from primary C57BL/6 mouse astrocyte cultures by using astrocyte medium (Cat #1801) conditioned for 48 hours. The medium is sterile filtered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.
33.) Yue X, Xu L, Zhu M, Zhang R, Liu C. (2013) "Over-expression of Nerve Growth Factor-α in human cholangiocarcinoma QBC939 cells promote tumor progression."?PLoS One. 8: e62024.
34.) Crowe A, Jackaman C, Beddoes KM, Ricciardo B, Nelson DJ. (2013) "Rapid copper acquisition by developing murine mesothelioma: decreasing bioavailable copper slows tumor growth, normalizes vessels and promotes T cell infiltration." PLoS One. 8: e73684.
35.) Zhao XC, Dou GR, Wang L, Liang L, Tian DM, Cao XL, Qin HY, Wang CM, Zhang P, Han H. (2013) "Inhibition of Tumor Angiogenesis and Tumor Growth by the DSL Domain of Human Delta-Like 1 Targeted to Vascular Endothelial Cells."?Neoplasia. 15 :815-25
36.) Lachance PA, Hazen A, Sevick-Muraca EM. (2013) "Lymphatic Vascular Response to Acute Inflammation."?PLoS One. 8: e76078.
37.) Aisha AF, Ismail Z, Abu-Salah KM, Siddiqui JM, Ghafar G, Abdul Majid AM. (2013) "Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice"?BMC Complement Altern Med. 13: 168.?
38.) Cheng HS, Sivachandran N, Lau A, Boudreau E, Zhao JL, Baltimore D, Delgado-Olguin P, Cybulsky MI, Fish JE. (2013) "MicroRNA-146 represses endothelial activation by inhibiting pro-inflammatory pathways."?EMBO Mol Med. 5: 1017-34.?
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文献和实验
33.) Yue X, Xu L, Zhu M, Zhang R, Liu C. (2013) "Over-expression of Nerve Growth Factor-α in human cholangiocarcinoma QBC939 cells promote tumor progression."?PLoS One. 8: e62024.
34.) Crowe A, Jackaman C, Beddoes KM, Ricciardo B, Nelson DJ. (2013) "Rapid copper acquisition by developing murine mesothelioma: decreasing bioavailable copper slows tumor growth, normalizes vessels and promotes T cell infiltration." PLoS One. 8: e73684.
35.) Zhao XC, Dou GR, Wang L, Liang L, Tian DM, Cao XL, Qin HY, Wang CM, Zhang P, Han H. (2013) "Inhibition of Tumor Angiogenesis and Tumor Growth by the DSL Domain of Human Delta-Like 1 Targeted to Vascular Endothelial Cells."?Neoplasia. 15 :815-25
36.) Lachance PA, Hazen A, Sevick-Muraca EM. (2013) "Lymphatic Vascular Response to Acute Inflammation."?PLoS One. 8: e76078.
37.) Aisha AF, Ismail Z, Abu-Salah KM, Siddiqui JM, Ghafar G, Abdul Majid AM. (2013) "Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice"?BMC Complement Altern Med. 13: 168.?
38.) Cheng HS, Sivachandran N, Lau A, Boudreau E, Zhao JL, Baltimore D, Delgado-Olguin P, Cybulsky MI, Fish JE. (2013) "MicroRNA-146 represses endothelial activation by inhibiting pro-inflammatory pathways."?EMBO Mol Med. 5: 1017-34.?
1、合适的细胞培养基合适的细胞培养基是体外细胞生长增殖的最重要的条件之一。培养基不仅提供细胞营养和促使细胞生长增殖的基础物质，而且还提供培养细胞生长和繁殖的生存环境。2、优质血清目前大多数合成培养基都需要添加血清。血清是细胞培养液中最重要的成分之一，含有细胞生长所需的多种生长因子及其它营养成分。3、无菌无毒细胞培养环境无菌无毒的操作环境和培养环境是保证细胞在体外培养成功的首要条件。在体外培养的细胞由于缺乏对微生物和有毒物的防御能力，一旦被微生物或有毒物质污染或者自身代谢物质积累，可导致细胞
一、 培养基的特性——细胞生存的环境与条件1、温度条件（37±0.5℃）2、pH条件 （7.2～7.4）3、气体条4、水的质量（三蒸）5、渗透压6、营养条件7、无菌条件二、 培养基的分类1、平衡盐液2、天然培养基（小牛血清、胚胎液）3、合成培养基（化学成分清楚）三、 RPMI1640培养基的配制1、RPMI1640 10g2、丙酮酸钠 0.11g3、Hepes（羟乙基哌碃乙硫磺酸）5.925g4、NaHCO3 2g5、三蒸水 1000ml电磁搅拌，过滤出菌，并进行无菌实验四
正常小鼠原代脑星形胶质细胞培养 一、实验试剂1、培养基： PriCells Medium + 10% FBS + 1% P/S + PriCells Supplement2、冻存液： PriCells Medium + 20% FBS + 10% DMSO3、洗涤液： 1 × PBS （pH 7.4 ）+ 1% P/S4、染色液： 0.4% Trypan Blue5、消化液： PriCells Isolation of Primary Cell Kit6、检测试剂：抗小鼠CD 11b/c抗体
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