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- Sciencell
- B-R1811
- 美国
- 2025年08月01日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
上海酶研生物科技有限公司
- 英文名：
RACM（Rat Astrocyte Conditioned Medium）,100ml
- 规格：
500ml/T
|
货号 |
R1811 |
|
产地 |
美国 |
|
缩写 |
RACM |
|
规格 |
100 ml |
|
用途 |
科研 |
|
储存 |
4度，-20度 |
|
运输 |
胶冰 |
Astrocytes are the most abundant cells in the central nervous system (CNS). Astrocytes provide a variety of biological functions, such as supporting endothelial cells involved in blood-brain barrier formation, providing nutrients to neurons, maintaining extracellular ion balance, and promoting repair in the CNS. Astrocyte conditioned medium (ACM) has been widely used to support the growth of neurons and endothelial cells in culture. ACM has also been shown to protect neurons from corticosterone-induced damage.
Rat ACM is a liquid medium prepared from primary rat astrocyte cultures by using astrocyte medium (Cat #1801) conditioned for 48 hours. The medium is sterile filtered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.
29.) Jandu SK, Webb AK, Pak A, Sevinc B, Nyhan D, Belkin AM, Flavahan NA, Berkowitz DE, Santhanam L. (2013) Nitric oxide regulates tissue transglutaminase localization and function in the vasculature.?Amino Acids. 44: 261-9.
30.) Cucullo L, Hossain M, Tierney M, Janigro D. (2013) "A new dynamic in vitro modular capillaries-venules modular system: cerebrovascular physiology in a box."?BMC Neurosci. 14: 18.
31.) Guo W, Zhang D, Wang X, Kong W, Zhang Q, Zhang Y, Liu W. (2013) "Actin cytoskeleton modulates ADMA-induced NF-kappaB nuclear translocation and ICAM-1 expression in endothelial cells."?Med Sci Monit. 17: BR242
32.) Tong X, Peng H, Liu D, Ji L, Niu C, Ren J, Pan B, Hu J, Zheng L, Huang Y. (2013) "High-density lipoprotein of patients with Type 2 Diabetes Mellitus upregulates cyclooxgenase-2 expression and prostacyclin I-2 release in endothelial cells: relationship with HDL-associated sphingosine-1-phosphate."?Cardiovasc Diabetol. 12: 27.
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文献和实验
29.) Jandu SK, Webb AK, Pak A, Sevinc B, Nyhan D, Belkin AM, Flavahan NA, Berkowitz DE, Santhanam L. (2013) Nitric oxide regulates tissue transglutaminase localization and function in the vasculature.?Amino Acids. 44: 261-9.
30.) Cucullo L, Hossain M, Tierney M, Janigro D. (2013) "A new dynamic in vitro modular capillaries-venules modular system: cerebrovascular physiology in a box."?BMC Neurosci. 14: 18.
31.) Guo W, Zhang D, Wang X, Kong W, Zhang Q, Zhang Y, Liu W. (2013) "Actin cytoskeleton modulates ADMA-induced NF-kappaB nuclear translocation and ICAM-1 expression in endothelial cells."?Med Sci Monit. 17: BR242
32.) Tong X, Peng H, Liu D, Ji L, Niu C, Ren J, Pan B, Hu J, Zheng L, Huang Y. (2013) "High-density lipoprotein of patients with Type 2 Diabetes Mellitus upregulates cyclooxgenase-2 expression and prostacyclin I-2 release in endothelial cells: relationship with HDL-associated sphingosine-1-phosphate."?Cardiovasc Diabetol. 12: 27.
1、合适的细胞培养基合适的细胞培养基是体外细胞生长增殖的最重要的条件之一。培养基不仅提供细胞营养和促使细胞生长增殖的基础物质，而且还提供培养细胞生长和繁殖的生存环境。2、优质血清目前大多数合成培养基都需要添加血清。血清是细胞培养液中最重要的成分之一，含有细胞生长所需的多种生长因子及其它营养成分。3、无菌无毒细胞培养环境无菌无毒的操作环境和培养环境是保证细胞在体外培养成功的首要条件。在体外培养的细胞由于缺乏对微生物和有毒物的防御能力，一旦被微生物或有毒物质污染或者自身代谢物质积累，可导致细胞
【摘要 】目的 诱导焦虑反应模型 ,观察焦虑反应对大鼠行为活动的影响。方法 条件恐惧训练 ,行为检测。结果 连续 4d的条件恐惧训练后 ,与对照组相比 , F344大鼠在木僵行为实验中木僵反应增多884% ( P 【关键词 】 恐惧; 条件反射; 焦虑; F344大鼠 Effect of cond iti oned fear tra i n i ng on behav i ora l acti v ity i n F344
一、 培养基的特性——细胞生存的环境与条件1、温度条件（37±0.5℃）2、pH条件 （7.2～7.4）3、气体条4、水的质量（三蒸）5、渗透压6、营养条件7、无菌条件二、 培养基的分类1、平衡盐液2、天然培养基（小牛血清、胚胎液）3、合成培养基（化学成分清楚）三、 RPMI1640培养基的配制1、RPMI1640 10g2、丙酮酸钠 0.11g3、Hepes（羟乙基哌碃乙硫磺酸）5.925g4、NaHCO3 2g5、三蒸水 1000ml电磁搅拌，过滤出菌，并进行无菌实验四
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