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- Sciencell
- B-1001-GF
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- 2025年08月01日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
上海酶研生物科技有限公司
- 英文名：
ECM-GF (Endothelial Cell Medium-GF)
- 规格：
500ml/T
|
1001-GF |
|
|
产地 |
北美 |
|
缩写 |
ECM-GF |
|
规格 |
500ml |
|
用途 |
科研 |
|
储存 |
4度，-20度 |
|
运输 |
胶冰 |
内皮细胞培养基-不含葡萄糖（ECM-GF）是为正常人类血管内皮细胞体外培养设计的适于其生长的完全培养基，需要添加内皮细胞生长添加物，血清和葡萄糖一起作用。该培养基是经灭菌的液体培养基，包含必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质和低浓度胎牛血清(5%)。该培养基缓冲体系为重碳酸盐，在含5%CO2的细胞培养箱中平衡后pH值为7.4。该培养基的配方能够选择性的促进正常人类血管内皮细胞体外培养中的增殖和生长，并为其达到理想营养平衡状态提供数量上和质量上的保证。
ECM-GF不含葡萄糖，葡萄糖是细胞生长中重要的能量来源，所以ECM-GF使用时需要添加葡萄糖（CS，货号#0893）。推荐葡萄糖浓度为1g/L，可根据实验要求调整葡萄糖浓度。
17.) Guo W, Ding J, Zhang A, Dai W, Liu S, Diao Z, Wang L, Han X, Liu W. (2014) "The Inhibitory Effect of Quercetin on Asymmetric Dimethylarginine-Induced Apoptosis Is Mediated by the Endoplasmic Reticulum Stress Pathway in Glomerular Endothelial Cells."?Int j mol sci. 15: 484-503.
18.) Lim PJ, Chu JJH. (2014) "A Polarized Cell Model for Chikungunya Virus Infection: Entry and Egress of Virus Occurs at the Apical Domain of Polarized Cells."?PLoSnegl trop dis. 8: e2661.
19.) Liu Q, Hou J, Chen X, Liu G, Zhang D, Sun H, Zhang J. (2014) "P-Glycoprotein Mediated Efflux Limits the Transport of the Novel Anti-Parkinson's Disease Candidate Drug FLZ across the Physiological and PD Pathological In Vitro BBB Models."?PloS one. 9: e102442.
20.) Tong X, Lv P, Mathew AV, Liu D, Niu C, Wang Y, Ji L, Li J, Fu Z, Pan B, Pennathur S, Zheng L, Huang Y. (2014) "The compensatory enrichment of sphingosine-1-phosphate harbored on glycated high-density lipoprotein restores endothelial protective function in type 2 diabetes mellitus."?Cardiovascdiabetol. 13: 82.
21.) Umar MI, Asmawi MZ, Sadikun A, Majid AMSA, Al-Suede FSR, Hassan LEA, Altaf R, Ahamed MBK. (2014) "Ethyl-p-methoxycinnamate isolated from kaempferiagalanga inhibits inflammation by suppressing interleukin-1, tumor necrosis factor-&aplha;, and angiogenesis by blocking endothelial functions."?Clinics. 69: 134-44.
22.) Voigt J, Christensen J, Shastri VP. (2014) "Differential uptake of nanoparticles by endothelial cells through polyelectrolytes with affinity for caveolae."?ProcNatlAcad Sci. 111: 2942-47.
23.) Wang Q, Yang J, Tang K, Luo L, Wang L, Tian L, Jiang Y, Feng J, Li Y, Shen B, Ly M, Huang Y. (2014) "Pharmacological characteristics and efficacy of a novel anti-angiogenic antibody FD006 in corneal neovascularization."?BMC biotechnol. 14: 17.
24.) Xing S, Yang X, Li W, Bian F, Wu D, Chi J, Xu G, Zhang Y, Jin S. (2014) "Salidroside Stimulates Mitochondrial Biogenesis and Protects against H 2 O 2-Induced Endothelial Dysfunction."?Oxid med cell longev. 2014: 904834.
25.) Xu Q, Xia P, Li X, Wang W, Liu Z, Gao X. (2014) "Tetramethylpyrazine Ameliorates High Glucose-Induced Endothelial Dysfunction by Increasing Mitochondrial Biogenesis."?PloS one. 9: e88243.
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文献和实验
17.) Guo W, Ding J, Zhang A, Dai W, Liu S, Diao Z, Wang L, Han X, Liu W. (2014) "The Inhibitory Effect of Quercetin on Asymmetric Dimethylarginine-Induced Apoptosis Is Mediated by the Endoplasmic Reticulum Stress Pathway in Glomerular Endothelial Cells."?Int j mol sci. 15: 484-503.
18.) Lim PJ, Chu JJH. (2014) "A Polarized Cell Model for Chikungunya Virus Infection: Entry and Egress of Virus Occurs at the Apical Domain of Polarized Cells."?PLoSnegl trop dis. 8: e2661.
19.) Liu Q, Hou J, Chen X, Liu G, Zhang D, Sun H, Zhang J. (2014) "P-Glycoprotein Mediated Efflux Limits the Transport of the Novel Anti-Parkinson's Disease Candidate Drug FLZ across the Physiological and PD Pathological In Vitro BBB Models."?PloS one. 9: e102442.
20.) Tong X, Lv P, Mathew AV, Liu D, Niu C, Wang Y, Ji L, Li J, Fu Z, Pan B, Pennathur S, Zheng L, Huang Y. (2014) "The compensatory enrichment of sphingosine-1-phosphate harbored on glycated high-density lipoprotein restores endothelial protective function in type 2 diabetes mellitus."?Cardiovascdiabetol. 13: 82.
21.) Umar MI, Asmawi MZ, Sadikun A, Majid AMSA, Al-Suede FSR, Hassan LEA, Altaf R, Ahamed MBK. (2014) "Ethyl-p-methoxycinnamate isolated from kaempferiagalanga inhibits inflammation by suppressing interleukin-1, tumor necrosis factor-&aplha;, and angiogenesis by blocking endothelial functions."?Clinics. 69: 134-44.
22.) Voigt J, Christensen J, Shastri VP. (2014) "Differential uptake of nanoparticles by endothelial cells through polyelectrolytes with affinity for caveolae."?ProcNatlAcad Sci. 111: 2942-47.
23.) Wang Q, Yang J, Tang K, Luo L, Wang L, Tian L, Jiang Y, Feng J, Li Y, Shen B, Ly M, Huang Y. (2014) "Pharmacological characteristics and efficacy of a novel anti-angiogenic antibody FD006 in corneal neovascularization."?BMC biotechnol. 14: 17.
24.) Xing S, Yang X, Li W, Bian F, Wu D, Chi J, Xu G, Zhang Y, Jin S. (2014) "Salidroside Stimulates Mitochondrial Biogenesis and Protects against H 2 O 2-Induced Endothelial Dysfunction."?Oxid med cell longev. 2014: 904834.
25.) Xu Q, Xia P, Li X, Wang W, Liu Z, Gao X. (2014) "Tetramethylpyrazine Ameliorates High Glucose-Induced Endothelial Dysfunction by Increasing Mitochondrial Biogenesis."?PloS one. 9: e88243.
无血清培养基(serum free medium, SFM)：是不需要添加血清就可以维持细胞在体外较长时间生长繁殖的合成培养基。简单地认为是体外细胞培养过程中的细胞培养基中不含有动物或人的血清，称为无血清细胞培养基。无血清培养基的基本配方是基本成分为基础培养基及添加组分两大部分。大多数的无血清培养基含有必须的向细胞内转运离子的转铁蛋白和调节葡萄糖摄取量的胰岛素，以及一些蛋白质如纤连蛋白、球蛋白、细胞生长因子等。无血清培养基被广泛的应用于培养哺乳动物和无脊动物细胞以制备单克隆抗体、病毒抗原、重组
) mimics，经检测在人类、小鼠、大鼠中均无同源片段，最大限度降低了对定量实验造成的干扰，适用于内参表达不稳定的外泌体或暂未发现合适内参的其他样品，对目的 microRNA 进行相对定量。外参序列：ucaccggguguaaaucagcuug。cel-miR-39-3p外参正序列为 AACACGCTCACCGGGTGTAA。 18、外泌体专用培养基是否可用于重悬、接种以及继续培养细胞？ A：不可以。外泌体专用培养基不含促细胞贴壁的因子，可以维持已贴壁的细胞一定时间内的生长，无法替代完全培养基长期
首个人类造血干细胞发育「路线图」发布！Nature 最新研究助力造血干细胞体外培育
from haemogenic endothelium to birth 为题发表在顶级期刊 Nature 上。 通过创建人类造血发生的单细胞转录组图谱，他们记录了 HSC 从动脉造血内皮细胞产生的全过程，并使用空间转录组学可视化主动脉内造血群（Intra-Aortic Haematopoietic Clusters, IAHCs）中 HSC 的产生。此外，通过比较不同成熟阶段的 HSC，建立了 HSC 出现后的转录因子机制，揭示了其表面分子标志物在整个发育过程中的不断演化。 通过建立人类 HSC 发育的详细
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