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- Sciencell-1811
- 美国
- 2025年08月01日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
上海酶研生物科技有限公司
- 英文名：
ACM ( Astrocyte conditioned Medium )---100ml
- 规格：
100ml/T
|
货号 |
1811 |
|
产地 |
美国 |
|
缩写 |
ACM |
|
规格 |
100ml |
|
用途 |
科研 |
|
储存 |
4度，-20度 |
|
运输 |
胶冰 |
星形胶质细胞是神经中枢最丰富的细胞。星形胶质细胞提供大量的生理功能，如支持内皮细胞作用于脑血管障碍形成，给神经元提供营养维持细胞外离子平衡，以及促进中枢神经系统的修复。星形胶质细胞条件培养基被广泛用于提供神经细胞和内皮细胞的培养。ACM表现出有保护神经细胞免于引起的损伤。
ACM是液体培养基，由原代人类星形胶质细胞在星形胶质细胞培养基（1801）条件下培养48小时得到。该培养基经过无菌过滤，在5%CO2的培养箱中达到7.4的PH值。
49.) Zhang S, Liu L, Wang R, Tuo H, Guo Y, Yi L, Wang D, Wang J. (2013) "MicroRNA-217 Promotes Angiogenesis of Human Cytomegalovirus-Infected Endothelial Cells through Downregulation of SIRT1 and FOXO3A."?PloS one. 8: e83620.
50.) Zhang W, Ni C, Sheng J, Hua Y, Ma J, Wang L, Zhao Y, Xing Y. (2013) "TLQP-21 Protects Human Umbilical Vein Endothelial Cells against High-Glucose-Induced Apoptosis by Increasing G6PD Expression."?PloS one. 8: e79760.
51.) Claessens A, Adams Y, Ghumra A, Lindergard G, Buchan CC, Andisi C, Bull PC, Mok S, Gupta AP, Wang CW, Turner L, Arman M, Raza A, Bozdech Z, Rowe JA. (2012) "A subset of group A-like var genes encodes the malaria parasite ligands for binding to human brain endothelial cells."?Proc Natl Acad Sci USA. 109: E1772-81.?
52.) Feng S, Cen J, Huang Y, Shen H, Yao L, Wang Y, Chen Z. (2011) "Matrix metalloproteinase-2 and -9 secreted by leukemic cells increase the permeability of blood-brain barrier by disrupting tight junction proteins."?PLoS One. 6: e20599.
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文献和实验
49.) Zhang S, Liu L, Wang R, Tuo H, Guo Y, Yi L, Wang D, Wang J. (2013) "MicroRNA-217 Promotes Angiogenesis of Human Cytomegalovirus-Infected Endothelial Cells through Downregulation of SIRT1 and FOXO3A."?PloS one. 8: e83620.
50.) Zhang W, Ni C, Sheng J, Hua Y, Ma J, Wang L, Zhao Y, Xing Y. (2013) "TLQP-21 Protects Human Umbilical Vein Endothelial Cells against High-Glucose-Induced Apoptosis by Increasing G6PD Expression."?PloS one. 8: e79760.
51.) Claessens A, Adams Y, Ghumra A, Lindergard G, Buchan CC, Andisi C, Bull PC, Mok S, Gupta AP, Wang CW, Turner L, Arman M, Raza A, Bozdech Z, Rowe JA. (2012) "A subset of group A-like var genes encodes the malaria parasite ligands for binding to human brain endothelial cells."?Proc Natl Acad Sci USA. 109: E1772-81.?
52.) Feng S, Cen J, Huang Y, Shen H, Yao L, Wang Y, Chen Z. (2011) "Matrix metalloproteinase-2 and -9 secreted by leukemic cells increase the permeability of blood-brain barrier by disrupting tight junction proteins."?PLoS One. 6: e20599.
Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
MEF-Conditioned Medium (MEF-CM) 1) Cover the whole surface of each new culture vessel with Attachment Factor (AF) solution and incubate the vessels for 30 minutes at 37°C or for 1 hour at room temperature. For MEF-CM generation, a T-175 flask
Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
Conditioned Medium to 37° C. Thaw the vial of BG01V hES cells by warming until just thawed and then immediately transfer to a 15 mL conical tube containing at least 5 mL of pre-warmed Human Feeder Cell Conditioned Medium. Spin
Production of Conditioned Medium containing Wingless protein from S2 cells
Production of Conditioned Medium containing Wingless protein from S2 cells - Grow heatshock wingless S2 cells at 25 degrees centigrade in a tissue culture flask to approximately 2.5 x 106 cells/ml. - Heat shock the cells at 37 degrees
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