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- Sciencell
- R1811
- 美国
- 2025年08月01日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
上海酶研生物科技有限公司
- 英文名：
RACM（Rat Astrocyte Conditioned Medium）,100ml
- 规格：
500ml/T
|
货号 |
R1811 |
|
产地 |
美国 |
|
缩写 |
RACM |
|
规格 |
100 ml |
|
用途 |
科研 |
|
储存 |
4度，-20度 |
|
运输 |
胶冰 |
Astrocytes are the most abundant cells in the central nervous system (CNS). Astrocytes provide a variety of biological functions, such as supporting endothelial cells involved in blood-brain barrier formation, providing nutrients to neurons, maintaining extracellular ion balance, and promoting repair in the CNS. Astrocyte conditioned medium (ACM) has been widely used to support the growth of neurons and endothelial cells in culture. ACM has also been shown to protect neurons from corticosterone-induced damage.
Rat ACM is a liquid medium prepared from primary rat astrocyte cultures by using astrocyte medium (Cat #1801) conditioned for 48 hours. The medium is sterile filtered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.
29.) Jandu SK, Webb AK, Pak A, Sevinc B, Nyhan D, Belkin AM, Flavahan NA, Berkowitz DE, Santhanam L. (2013) Nitric oxide regulates tissue transglutaminase localization and function in the vasculature.?Amino Acids. 44: 261-9.
30.) Cucullo L, Hossain M, Tierney M, Janigro D. (2013) "A new dynamic in vitro modular capillaries-venules modular system: cerebrovascular physiology in a box."?BMC Neurosci. 14: 18.
31.) Guo W, Zhang D, Wang X, Kong W, Zhang Q, Zhang Y, Liu W. (2013) "Actin cytoskeleton modulates ADMA-induced NF-kappaB nuclear translocation and ICAM-1 expression in endothelial cells."?Med Sci Monit. 17: BR242
32.) Tong X, Peng H, Liu D, Ji L, Niu C, Ren J, Pan B, Hu J, Zheng L, Huang Y. (2013) "High-density lipoprotein of patients with Type 2 Diabetes Mellitus upregulates cyclooxgenase-2 expression and prostacyclin I-2 release in endothelial cells: relationship with HDL-associated sphingosine-1-phosphate."?Cardiovasc Diabetol. 12: 27.
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文献和实验
29.) Jandu SK, Webb AK, Pak A, Sevinc B, Nyhan D, Belkin AM, Flavahan NA, Berkowitz DE, Santhanam L. (2013) Nitric oxide regulates tissue transglutaminase localization and function in the vasculature.?Amino Acids. 44: 261-9.
30.) Cucullo L, Hossain M, Tierney M, Janigro D. (2013) "A new dynamic in vitro modular capillaries-venules modular system: cerebrovascular physiology in a box."?BMC Neurosci. 14: 18.
31.) Guo W, Zhang D, Wang X, Kong W, Zhang Q, Zhang Y, Liu W. (2013) "Actin cytoskeleton modulates ADMA-induced NF-kappaB nuclear translocation and ICAM-1 expression in endothelial cells."?Med Sci Monit. 17: BR242
32.) Tong X, Peng H, Liu D, Ji L, Niu C, Ren J, Pan B, Hu J, Zheng L, Huang Y. (2013) "High-density lipoprotein of patients with Type 2 Diabetes Mellitus upregulates cyclooxgenase-2 expression and prostacyclin I-2 release in endothelial cells: relationship with HDL-associated sphingosine-1-phosphate."?Cardiovasc Diabetol. 12: 27.
Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
MEF-Conditioned Medium (MEF-CM) 1) Cover the whole surface of each new culture vessel with Attachment Factor (AF) solution and incubate the vessels for 30 minutes at 37°C or for 1 hour at room temperature. For MEF-CM generation, a T-175 flask
胶质细胞培养（astrocyte, oligodendrocyte）
取材及胶质细胞的混合培养1、P2 SD大鼠经低温麻醉后以碘酒和75％乙醇消毒，无菌操作下，断头放入预冷的D-Hanks液中，在解剖显微镜下取大脑皮层并除去脑膜。2、剪碎组织成1mm3大小，加入胰酶-EDTA消化液并放入37℃孵箱内消化20min，中间摇晃一次。3、随后用滴管吸出组织转移到装有预冷的MEM＋10％FBS（Glial Medium，GM）的离心管内终止消化液作用5min。4、吸出组织转移到另一支装有冷的GM的离心管内，用火焰抛光的巴斯德滴管吹打数次，静置后取上清并吸到另一
Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
Conditioned Medium to 37° C. Thaw the vial of BG01V hES cells by warming until just thawed and then immediately transfer to a 15 mL conical tube containing at least 5 mL of pre-warmed Human Feeder Cell Conditioned Medium. Spin
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