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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C
- 保质期:
6个月
- 库存:
100
- 供应商:
百欧泰
- 规格:
5ml/10ml
| 规格: | 5ml | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 10ml | 产品价格: | ¥2500.0 |
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文献和实验- make a 5 uM stock solution of adaptors - add 5 ul of the top strand adaptor (100 uM) and 5 ul of the bottom strand adaptor (100 uM) to 80 ul H2O + 10 ul 10X BRL buffer 3. c) heat both to boiling, and let cool to RT. Ligation of Adaptors
ELISPOT (Enzyme-linked ImmunoSPOT)
cytokine. The detected cytokine is then visualized using avidin-HRP, and a precipitating substrate (e.g., AEC). The colored end product (spot) represents an individual cytokine-producing cell. The spots can be counted manually (e.g., with a dissecting
ml warm D10 onto plate and place in incubator. 36-48 hours after transfection, harvest viral supernatant and spin at 2000 rpm, 7 min at 4� in a 50ml tube. Filter viral SN through .45 um filter. Add 35ml of filtered
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