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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C
- 保质期:
6个月
- 库存:
100
- 供应商:
百欧泰
- 规格:
5ml/10ml
| 规格: | 5ml | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 10ml | 产品价格: | ¥2500.0 |
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文献和实验solution)to microcentrifuge tube. 2.Add 2.5x 10,000 particles of 1 uM Fluosphere beads. 3.Mix overnight at 4 ℃ with 200 µl 5mg/ml 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. 4.Wash with PBS,by centrifuging,repeat wash 3 times. 5.Resuspend the bead
ganglioside (diluted in PBS from 5mM in DMSO stocking solution) to microcentrifuge tube. Add 2.5x 10,000 particles of 1 uM Fluosphere beads. Mix overnight at 4 o C with 200 µl 5mg/ml
= 5’GCCTCCCTCGCGCCATCAGTATCGTAGGCACCTGAGASequence of 3’ primer= 5’AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA/iSp18/GCCTTGCCAGCCCGCTCAGTATTGATGGTGCCTACAG(purchased from Integrated DNA Technologies, Inc)0.75 uL100 uM 5’ primer0.75 uL100 uM 3’ primer10 uL10X PCR buffer (1X = 10 mM TRIS, pH8.4, 50 mM KCl, 1.5 mM
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