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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期:
1 year
- 英文名:
Recombinant Mouse BRD4 Protein, N-GST & C-His
- 库存:
999
- 供应商:
abinScience
- 规格:
100ug

| Product name | Recombinant Mouse BRD4 Protein, N-GST & C-His |
|---|---|
| Catalog No. | MF757012 |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | E. coli |
| Accession | Q9ESU6 |
| Reconstitution | Reconstitute in sterile water for a stock solution. |
| Alternative Names | Bromodomain-containing protein 4, Mitotic chromosome-associated protein, MCAP, Brd4 |
| Species | Mus musculus (Mouse) |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
abinScience, founded in 2023 in Strasbourg, France, is committed to developing and producing high-quality life science reagents. Rooted in one of Europe’s leading hubs for scientific innovation, abinScience empowers global research through reliable, efficient experimental solutions. Guided by the vision of “Empowering Bioscience Discovery,” we support scientists worldwide in advancing the frontiers of bioscience.
Technology and Innovation
Relying on the global leading position in antibody and protein research and more than 20 years of industry experience of its parent company ProteoGenix since 2003...
Product categories and application fields.
Main products...
Application field: Infectious disease research, Neuroscience, Immunology, Oncology
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文献和实验• BRD4: New hope in the battle against glioblastoma., PMID:37061146
• Targeting BRD4 prevents acute gouty arthritis by regulating pyroptosis., PMID:33162822
• Hijacking the E3 Ubiquitin Ligase Cereblon to Efficiently Target BRD4., PMID:26051217
• Supercharging BRD4 with NUT in carcinoma., PMID:33452461
• BRD4 as a potential target for human papillomaviruses associated cancer., PMID:38100650
• Regulation of programmed cell death by Brd4., PMID:36539410
• Apoptotic body-inspired nanotherapeutics efficiently attenuate osteoarthritis by targeting BRD4-regulated synovial macrophage polarization., PMID:38330742
• BRD4 as a Therapeutic Target in Pulmonary Diseases., PMID:37686037
• BRD4 Protein as a Target for Lung Cancer and Hematological Cancer Therapy: A Review., PMID:37846578
depends on product and its function. N-terminal sequencing revealed that the purified rBoNTC(Hc )-N is missing the first eight amino acids of the N-terminus of the protein, whereas the purified rBoNTC(Hc )-C protein is intact. After a mouse bioassay test
Thrombin Cleavage of GST-Fusion protein
activity keeps the enzyme in aliquots at -80°C. Thrombin (MW 37kDa) ~4500units/mg. One unit cleaves theoretical 100ug protein in 16hr at 22°C in buffer. Another possibility is to use high purity enzyme from Sigma # T-6884: 3-10 U of thrombin can be used
5- If required, bring to 1pM RARE or any HRE you need 6- Incubate 30min at 4 _ C 7- Add 50_ L of the GST affinity matrix 8- Incubate for 2 hours at 4 _ C 9- Wash twice with PBS-DTT buffer (quick pulse to 500 x g) 10- Add 25_ L of SDS loading buffer directly
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