- ￥1868
- abinScience
- France
- HW825011
- 2026年03月03日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期：
1 year
- 英文名：
Recombinant Human Hyaluronidase PH-20 Protein
- 库存：
999
- 供应商：
abinScience
- 规格：
100ug
| Product name | Recombinant Human Hyaluronidase PH-20 Protein |
|---|---|
| Catalog No. | HW825011 |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | Mammalian Cells |
| Accession | P38567 |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Alternative Names | Hyaluronidase PH-20, Hyal-PH20, EC:3.2.1.35, Hyaluronoglucosaminidase PH-20, Sperm adhesion molecule 1, Sperm surface protein PH-20, SPAM1, HYAL3, PH20 |
| Species | Homo sapiens (Human) |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
abinScience, founded in 2023 in Strasbourg, France, is committed to developing and producing high-quality life science reagents. Rooted in one of Europe’s leading hubs for scientific innovation, abinScience empowers global research through reliable, efficient experimental solutions. Guided by the vision of “Empowering Bioscience Discovery,” we support scientists worldwide in advancing the frontiers of bioscience.
Technology and Innovation
Relying on the global leading position in antibody and protein research and more than 20 years of industry experience of its parent company ProteoGenix since 2003...
Product categories and application fields.
Main products...
Application field: Infectious disease research, Neuroscience, Immunology, Oncology
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文献和实验• The dual functions of GPI-anchored PH-20: hyaluronidase and intracellular signaling., PMID:11731269
• The human sperm protein PH-20 has hyaluronidase activity., PMID:8282124
• Recombinant human hyaluronidase (rHuPH20): an enabling platform for subcutaneous drug and fluid administration., PMID:17683255
• ENHANZE® drug delivery technology: a novel approach to subcutaneous administration using recombinant human hyaluronidase PH20., PMID:30744432
• Structural relationship of sperm soluble hyaluronidase to the sperm membrane protein PH-20., PMID:8724363
• Importance of glycosylation and disulfide bonds in hyaluronidase activity of macaque sperm surface PH-20., PMID:11868814
• Enzymatic targeting of the stroma ablates physical barriers to treatment of pancreatic ductal adenocarcinoma., PMID:22439937
• The PH-20 protein in cynomolgus macaque spermatozoa: identification of two different forms exhibiting hyaluronidase activity., PMID:8608861
• The soluble hyaluronidase from bull testes is a fragment of the membrane-bound PH-20 enzyme., PMID:9280317
DETECTION OF RECOMBINANT PROTEINS BY ELISA
of interest. If the recombinant protein is not soluble in H2 O, dissolve the insoluble pellet in a volume of 7 M urea equal to 1/10 of the bacterial culture volume. Vortex the samples at a medium speed for about 30 minutes (use a vortex setting of 4.5 on the Fisher Vortex Genie
【原创】Protein A Sepharose 之进化篇。。。
Protein A的其他非结合域会和非目标蛋白结合，这样被洗脱下来的蛋白质纯度不够，会影响到后续的试验。 为了解决这些问题，科学家们运用基因工程技术，克隆出Protein A的基因，并对其进行改造，除去了一些不重要的非结合域。偶联这种重组Protein A的琼脂糖凝胶柱在蛋白质纯化中，的确是提高了产物的纯度。目前，市场上绝大部分重组Protein A柱都是这种产品。但是，纯化时所用的洗脱液一般为pH=2.7的甘氨酸溶液，如果洗脱效果不是很理想，还要降低pH，采用pH=1.9的甘氨酸溶液。由此可见，此法
Purification of recombinant sBRF M166L
the elution and freeze samples. Analyze recovery by SDS PAGE and/or protein assay. Typical recovery is 2 to 6 mg BRF protein. Lysis/Binding buffer, pH 8.0 (150 ml) 30mM Tris 8.0 0.54 g Tris
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