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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期:
1 year
- 英文名:
Recombinant Human S100A5 Protein, N-GST & C-His
- 库存:
999
- 供应商:
abinScience
- 规格:
100ug

| Product name | Recombinant Human S100A5 Protein, N-GST & C-His |
|---|---|
| Catalog No. | HW627012 |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | E. coli |
| Accession | P33763 |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Alternative Names | Protein S100-A5; Protein S-100D; S100 calcium-binding protein A5; S100A5; S100D |
| Species | Homo sapiens (Human) |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
abinScience, founded in 2023 in Strasbourg, France, is committed to developing and producing high-quality life science reagents. Rooted in one of Europe’s leading hubs for scientific innovation, abinScience empowers global research through reliable, efficient experimental solutions. Guided by the vision of “Empowering Bioscience Discovery,” we support scientists worldwide in advancing the frontiers of bioscience.
Technology and Innovation
Relying on the global leading position in antibody and protein research and more than 20 years of industry experience of its parent company ProteoGenix since 2003...
Product categories and application fields.
Main products...
Application field: Infectious disease research, Neuroscience, Immunology, Oncology
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文献和实验• Human S100A5 binds Ca2+ and Cu2+ independently., PMID:29201357
• S100A5: a marker of recurrence in WHO grade I meningiomas., PMID:15043715
• Brain S100A5 is a novel calcium-, zinc-, and copper ion-binding protein of the EF-hand superfamily., PMID:10882717
• Biophysical characterization of Ca2+-binding of S100A5 and Ca2+-induced interaction with RAGE., PMID:28017722
• Pentamidine blocks the interaction between mutant S100A5 and RAGE V domain and inhibits the RAGE signaling pathway., PMID:27297108
• Were Ancestral Proteins Less Specific?, PMID:33528559
• Quantitative Proteomics Reveal an Altered Pattern of Protein Expression in Brain Tissue from Mice Lacking GPR37 and GPR37L1., PMID:31903766
• Restricted expression of calcium-binding protein S100A5 in human kidney., PMID:11855835
• Meningioma: an update., PMID:15542977
Thrombin Cleavage of GST-Fusion protein
activity keeps the enzyme in aliquots at -80°C. Thrombin (MW 37kDa) ~4500units/mg. One unit cleaves theoretical 100ug protein in 16hr at 22°C in buffer. Another possibility is to use high purity enzyme from Sigma # T-6884: 3-10 U of thrombin can be used
Small Scale GST-Tag Purification Under Nature Conditions
Small Scale GST-Tag Purification Under Nature Conditions According to AMERSHAM-BIOSCIENCES-GST fusion protein Handbook (pdf) Aliquot of Cell Pellet after Induction The idea is to aliquot cells after induction
High Throughput Production of Recombinant Human Proteins for Crystallography
, to be applied based on specific knowledge of protein families or when the core protocol is unsatisfactory. This scheme has been applied to more than 550 human proteins (x003E;10,000 constructs) and has resulted in the deposition of 112 unique structures
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