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- abinScience
- France
- HP872012
- 2026年03月03日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期：
1 year
- 英文名：
Recombinant Human EMC10 Protein, N-His
- 库存：
999
- 供应商：
abinScience
- 规格：
100ug
| Product name | Recombinant Human EMC10 Protein, N-His |
|---|---|
| Catalog No. | HP872012 |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | E. coli |
| Accession | Q5UCC4 |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Alternative Names | ER membrane protein complex subunit 10; Hematopoietic signal peptide-containing membrane domain-containing protein 1; EMC10; C19orf63; INM02; UNQ764/PRO1556 |
| Species | Homo sapiens (Human) |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
abinScience, founded in 2023 in Strasbourg, France, is committed to developing and producing high-quality life science reagents. Rooted in one of Europe’s leading hubs for scientific innovation, abinScience empowers global research through reliable, efficient experimental solutions. Guided by the vision of “Empowering Bioscience Discovery,” we support scientists worldwide in advancing the frontiers of bioscience.
Technology and Innovation
Relying on the global leading position in antibody and protein research and more than 20 years of industry experience of its parent company ProteoGenix since 2003...
Product categories and application fields.
Main products...
Application field: Infectious disease research, Neuroscience, Immunology, Oncology
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文献和实验• Secreted EMC10 is upregulated in human obesity and its neutralizing antibody prevents diet-induced obesity in mice., PMID:36443308
• Biallelic loss of EMC10 leads to mild to severe intellectual disability., PMID:35684946
• Membrane-Bound EMC10 Is Required for Sperm Motility via Maintaining the Homeostasis of Cytoplasm Sodium in Sperm., PMID:36077468
• EMC10 governs male fertility via maintaining sperm ion balance., PMID:29659949
• The phenotype of homozygous EMC10 variant: A new syndrome with intellectual disability and language impairment., PMID:35124540
• A recurrent, homozygous EMC10 frameshift variant is associated with a syndrome of developmental delay with variable seizures and dysmorphic features., PMID:33531666
• EMC10 homozygous variant identified in a family with global developmental delay, mild intellectual disability, and speech delay., PMID:32869858
• EMC10 (Endoplasmic Reticulum Membrane Protein Complex Subunit 10) Is a Bone Marrow-Derived Angiogenic Growth Factor Promoting Tissue Repair After Myocardial Infarction., PMID:28931551
干货 | CUT and Tag 核心酶 Hyperactive PA/PG-Tn5 Transposase 大解密
CUT&Tag（Cleavage Under Targets and Tagmentation）是蛋白-DNA 互作的一大革新技术，它不需要使用甲醛交联以及免疫共沉淀，而是通过针对靶蛋白（如转录因子、染色质重塑蛋白）的抗体和 Protein A/G 的介导，使得与 Protein A/G 融合的 Tn5 酶(Tagmentase)在切割 DNA 片段的同时在序列两端加上测序接头，经 PCR 扩增后即可形成用于高通量测序的文库（见图 1 ）。CUT &Tag 技术具有细胞投入量低、信噪
的分离速度，但是附加PA10时分离速度有明显加快，KD値也会增大。 亲和标记法的确立使用单克隆抗体和肽标记的亲和标签系统有很多要求，具体来说要求如下：1）高特异性，能从众多杂质中得到目的蛋白；2）高亲和性，结合了的标记蛋白不会马上分离；3）能够在洗脱时保持蛋白活性；4）为了降低成本，色谱柱希望能多次循环再生使用。等等，能满足以上条件，就是一个好的亲和标签系统。首先，作为NZ-1的抗原使用的14个氨基酸残基的肽需要决定最短长度，长度越短使用越方便，对附加tag的蛋白的影响也越小。从加藤等人以前使用
High Throughput Production of Recombinant Human Proteins for Crystallography
This chapter presents in detail the process used in high throughput bacterial production of recombinant human proteins for crystal structure determination. The core principles are: (1 ) Generating at least 10 truncated constructs
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