Recombinant Human POLR2K Prote
in (N-GST & C-His Tag), 重组人POLR2K蛋白 (N端GST标签 & C端His标签)- ￥1868
- abinScience
- France
- HA717012
- 2026年03月03日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期：
1 year
- 英文名：
Recombinant Human POLR2K Protein, N-GST & C-His
- 库存：
999
- 供应商：
abinScience
- 规格：
100ug
| Product name | Recombinant Human POLR2K Protein, N-GST & C-His |
|---|---|
| Catalog No. | HA717012 |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | E. coli |
| Accession | P53803 |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Alternative Names | DNA-directed RNA polymerases I, II, and III subunit RPABC4; RNA polymerases I, II, and III subunit ABC4; ABC10-alpha; DNA-directed RNA polymerase II subunit K; RNA polymerase II 7.0 kDa subunit; RPB7.0; RPB10alpha; POLR2K |
| Species | Homo sapiens (Human) |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
abinScience, founded in 2023 in Strasbourg, France, is committed to developing and producing high-quality life science reagents. Rooted in one of Europe’s leading hubs for scientific innovation, abinScience empowers global research through reliable, efficient experimental solutions. Guided by the vision of “Empowering Bioscience Discovery,” we support scientists worldwide in advancing the frontiers of bioscience.
Technology and Innovation
Relying on the global leading position in antibody and protein research and more than 20 years of industry experience of its parent company ProteoGenix since 2003...
Product categories and application fields.
Main products...
Application field: Infectious disease research, Neuroscience, Immunology, Oncology
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文献和实验• An integrated co-expression network analysis reveals novel genetic biomarkers for immune cell infiltration in chronic kidney disease., PMID:36875100
• Integrated analysis of single-cell RNA sequencing and bulk transcriptome data identifies a pyroptosis-associated diagnostic model for Parkinson's disease., PMID:39558055
• Reprogramming barriers in bovine cells nuclear transfer revealed by single-cell RNA-seq analysis., PMID:35971640
• An in-depth map of polyadenylation sites in cancer., PMID:22753024
• Identification of miRNA biomarkers of pneumonia using RNA-sequencing and bioinformatics analysis., PMID:28413462
• Chlorogenic acid prevents acetaminophen-induced liver injury: the involvement of CYP450 metabolic enzymes and some antioxidant signals., PMID:26160718
• Identification of key pathways, genes and immune cell infiltration in hypoxia of high-altitude acclimatization via meta-analysis and integrated bioinformatics analysis., PMID:37035734
• Identification of key gene modules and hub genes of human mantle cell lymphoma by coexpression network analysis., PMID:32219041
• Pan-cancer integrated bioinformatic analysis of RNA polymerase subunits reveal RNA Pol I member CD3EAP regulates cell growth by modulating autophagy., PMID:37795959
Thrombin Cleavage of GST-Fusion protein
activity keeps the enzyme in aliquots at -80°C. Thrombin (MW 37kDa) ~4500units/mg. One unit cleaves theoretical 100ug protein in 16hr at 22°C in buffer. Another possibility is to use high purity enzyme from Sigma # T-6884: 3-10 U of thrombin can be used
Small Scale GST-Tag Purification Under Nature Conditions
Small Scale GST-Tag Purification Under Nature Conditions According to AMERSHAM-BIOSCIENCES-GST fusion protein Handbook (pdf) Aliquot of Cell Pellet after Induction The idea is to aliquot cells after induction
High Throughput Production of Recombinant Human Proteins for Crystallography
, to be applied based on specific knowledge of protein families or when the core protocol is unsatisfactory. This scheme has been applied to more than 550 human proteins (>10,000 constructs) and has resulted in the deposition of 112 unique structures
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