- ￥1868
- abinScience
- France
- HB341012
- 2026年03月03日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期：
1 year
- 英文名：
Recombinant Human HCLS1 Protein, N-GST & C-His
- 库存：
999
- 供应商：
abinScience
- 规格：
100ug
| Product name | Recombinant Human HCLS1 Protein, N-GST & C-His |
|---|---|
| Catalog No. | HB341012 |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | E. coli |
| Accession | P14317 |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Alternative Names | Hematopoietic lineage cell-specific protein; Hematopoietic cell-specific LYN substrate 1; LckBP1; p75; HCLS1; HS1 |
| Species | Homo sapiens (Human) |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
abinScience, founded in 2023 in Strasbourg, France, is committed to developing and producing high-quality life science reagents. Rooted in one of Europe’s leading hubs for scientific innovation, abinScience empowers global research through reliable, efficient experimental solutions. Guided by the vision of “Empowering Bioscience Discovery,” we support scientists worldwide in advancing the frontiers of bioscience.
Technology and Innovation
Relying on the global leading position in antibody and protein research and more than 20 years of industry experience of its parent company ProteoGenix since 2003...
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Application field: Infectious disease research, Neuroscience, Immunology, Oncology
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文献和实验• Hematopoietic cell-specific lyn substrate (HCLS1 or HS1): A versatile actin-binding protein in leukocytes., PMID:30537294
• Correlation between elevated HCLS1 levels and heart failure: A diagnostic biomarker., PMID:38847679
• Energy stress promotes P-bodies formation via lysine-63-linked polyubiquitination of HAX1., PMID:38769438
• Interactions among HCLS1, HAX1 and LEF-1 proteins are essential for G-CSF-triggered granulopoiesis., PMID:23001182
• Comprehensive analysis identifies DNA damage repair-related gene HCLS1 associated with good prognosis in lung adenocarcinoma., PMID:37969376
• Human Cytomegalovirus Upregulates Expression of HCLS1 Resulting in Increased Cell Motility and Transendothelial Migration during Latency., PMID:31569051
• Hsa-miR223-3p circulating level is upregulated in Friedreich's ataxia and inversely associated with HCLS1 associated protein X-1, HAX-1., PMID:35015850
• Identification and functional characterization of hCLS1, a human cardiolipin synthase localized in mitochondria., PMID:16716149
• Cloning and characterization of a cDNA encoding human cardiolipin synthase (hCLS1)., PMID:16547353
Small Scale GST-Tag Purification Under Nature Conditions
Small Scale GST-Tag Purification Under Nature Conditions According to AMERSHAM-BIOSCIENCES-GST fusion protein Handbook (pdf) Aliquot of Cell Pellet after Induction The idea is to aliquot cells after induction
-S10 柱子对于去污剂有高度的结合容量，比如每毫升固定的树脂可以结合 6 mg SDS 和 14 mg Triton® X-100。Table 1: A comparison of the detergent removal rates and percentage protein recovery with DetergentOUT™ GBS10. ag 蛋白的纯化 带有融合标签的表达产物----融合蛋白的纯化通常也是紧接细胞破碎工作进行的。设计融合标签的主要目的之一就是为了方便
[资源][原创] 蛋白标签纯化技术——CBP（钙调蛋白结合肽）
缓冲液中能够特异性的被钙调蛋白树脂捕捉吸附，并且在中性环境中能够被2 mM EGTA洗脱，这与6组氨酸亲合标签纯化体系相比反应条件要温和许多。仅有4-kDa 大小的CBP tag 与26-kDa GST 标签相比对蛋白分子的影响非常小。在所表达的蛋白分子上包含一个凝血酶或肠激酶列解位点，依靠它可以非常方便的移除CBP标签。在哺乳动物细胞中表达的标签纯化系统(两步纯化法用于高纯度的要求)Mammalian TAP System本系统同时用到了streptavidin binding peptide
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