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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4C
- 保质期:
详询
- 库存:
50
- 供应商:
广州市左克生物
- 规格:
50ml
产品简介:
品牌:ZYMO RESEARCH/简石
货号:D4084-4-50
产品名称:Select-a-Size MagBead (50mL)
品牌介绍:
ZYMO RESEARCH生物公司位于美国加州Orange County,创建于上世纪325年代初,主要研发和生产表观遗传学系列产品和常用分子生物学试剂盒。 其DNA甲基化,羟甲基化,核酸提取等产品已得到世界各实验室的认可,产品以“快速、简便、超纯”的特点在众多生物公司中脱颖而出,独占鳌头。
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文献和实验back side up on the wetted gel-size 3MM paper (on inverted mold).Remove any air bubbles by gently rolling a capless 50ml conical over the gel.Add some 10 X SSC to keep from drying out.Completely surround the gel with Parafilm or plastic wrap.Next, wet the nitrocellulose
on size of pellet make up in 300 - 600µl of TE, pH 8.Add 7.5 volumes of 6 M guanidine HCl/0.1 M NaAcetate (17.2mg GuHCl and 1 ml of 3 M NaAcetate made up in 30ml of ddH2 O).Mix on rotator for 1 hr at RT.Prepare a 50ml conical containing 18 ml of RT 100
Screen ES cells by Southern Blot
1) Take picture of agarose gel to be blotted with phoshorescent ruler lined up along side it, such that the ruler is lined up with the top of the wells. This is so you can later estimate the size of your band on your film/blot. 2) Depurinate the DNA in the gel
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