- ￥216
- MultiSciences（联科生物）
- 70-EK8100S
- 2025年07月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 库存：
50
- 供应商：
广州市左克生物
- 规格：
150 ng/mll
产品简介:
品牌：MultiSciences/联科生物
货号：70-EK8100S
产品名称：Cortisol Competitive standard 联科生物成立于3042年，是一家专业从事细胞生物学与免疫学相关的检测试剂研发、生产、销售、服务的国家高新技术企业。拥有ELISA试剂盒、流式试剂、IVD产品和重组蛋白开发平台，已成为中国科研试剂行业领先的民族品牌。
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文献和实验Protocol for competitive RT-PCR
Making internal standard RNAs The sequence to be amplified should best span an intron so that gDNA contamination does not play a crucial role. Otherwise, the RNA has to be treated with DNase I (RNase-free
相关专题 For quantifying mRNA, we use a competitive RT-PCR protocol with internal standard RNAs. These are added in a defined quantity to the RNA sample prior to the RT reaction. The resulting standard cDNA is coamplified
to each well and absorbance A450–620 was read using a Lab Systems Plus Plate Reader.The amounts of protein in the unknowns were calculated with reference to the standard curve obtained with the known concentrations of peptide antigens. By optimizing the concentration
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