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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Human PD1 / PDCD1 / CD279 Protein (Fc Tag), Endotoxin-Free
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100.00 µg/1.00 mg
| 规格: | 100.00 µg | 产品价格: | ¥3490.0 |
|---|---|---|---|
| 规格: | 1.00 mg | 产品价格: | ¥22670.0 |
蛋白名称:重组人PD1 / PDCD1 / CD279蛋白 (Fc标签)
蛋白构建:A DNA sequence encoding the human PDCD1 (NP_005009.2) (Met1-Gln167) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS(Routinely tested).
蛋白活性:1.Loaded Recombinant Human PD-1 Protein (ECD, hFc Tag), HPLC-verified (Cat. No. 10377-H02H-UE) on proA Biosensor, can bind Recombinant Human PD-L1 / B7-H1 / CD274 Protein (His Tag) (Cat. No. 10084-H08H) with an affinity constant of 1.78 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 2.Loaded Recombinant Human PD-1 Protein (ECD, hFc Tag), HPLC-verified (Cat. No. 10377-H02H-UE) on proA Biosensor, can bind Recombinant Human B7-DC / PD-L2 / CD273 Protein (His Tag) , Biotinylated (Cat. No. 10292-H08H-B) with an affinity constant of 0.227 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 3.Recombinant Human PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat: 10377-H02H-UE) captured on Protein A chip, can bind Recombinant Human PD-L1 / B7-H1 / CD274 Protein (His Tag) (Cat: 10084-H08H) with an affinity constant of 1.850 μM as determined in an SPR assay (Biacore T200) (Routinely tested). 4.Recombinant Human B7-DC / PD-L2 / CD273 Protein (His Tag) (Cat: 10292-H08H) captured on CM5 chip via Anti-His antibody, can bind Recombinant Human PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat: 10377-H02H-UE) with an affinity constant of 13.600 nM as determined in an SPR assay (Biacore 8K) (Routinely tested).
蛋白内毒素:< 0.5 EU per mg protein.
预测N端:Leu 25
蛋白分子量:The recombinant human PDCD1 consists of 381 amino acids and predicts a molecular mass of 42.7 kDa. As a result of glycosylation, the band migrates approximately 65-75 kDa in the SDS-PAGE under reducing conditions. The molecular weight of this protein is around 111.3 kDa verified by SEC-MALS(Routinely tested).
蛋白NP号:NP_005009.2
蛋白氨基酸序列:Met1-Gln167
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下调不能说明活性降低,因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外,还要做磷酸化的GSK3-beta的蛋白表达。 (2)应该活性形式和非活性形式都做。一般来讲,GSK3-beta在Ser9位点磷酸化之后活性收到抑制,而在216位点磷酸化之后,其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了,另外还要同时检测GSK3-beta的总蛋白表达,这样才能全面的说明问题。
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