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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Human Transferrin Receptor / TFRC / CD71 Protein (ECD, His Tag), Endotoxin-Free
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100.00 µg/1.00 mg
| 规格: | 100.00 µg | 产品价格: | ¥3490.0 |
|---|---|---|---|
| 规格: | 1.00 mg | 产品价格: | ¥26170.0 |
蛋白名称:重组人Transferrin Receptor / TFRC / CD71蛋白(胞外区, His标签)
蛋白构建:A DNA sequence encoding the extracellular domain of human TFRC (AAA61153.1) (Cys89-Phe760) was expressed with a polyhistidine tag at the N-terminus.
表达宿主:HEK293 Cells
蛋白纯度:≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性:Immobilized Recombinant Human Transferrin Receptor / TFRC / CD71 Protein (ECD, His Tag)(Cat: 11020-H07H-UE) at 2 μg/mL (100 μL/well) can bind Recombinant Human Transferrin Protein, Biotinylated (Cat: 11019-HNAH2-B), the EC50 is 10 ng/mL (Routinely tested).
蛋白内毒素:< 0.5 EU per mg protein.
预测N端:His
蛋白分子量:The recombinant Human TFRC comprises 688 amino acids and has a predicted molecular mass of 77.37 kDa. The bands migrate approximately 75 kDa and 150 kDa in the SDS-PAGE under reducing conditions.
蛋白NP号:AAA61153.1
蛋白氨基酸序列:Cys89-Phe760
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文献和实验Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
the isolation of activated Fc receptor complexes from RAW 264.7 macrophages using live?cell affinity receptor chromatography (LARC). The Fc receptor complex is activated and captured by IgG?coated microbeads on the surface of live macrophages. After the cells
特异性的引物PCR扩增。基因经过30-35个循环的一轮PCR扩增。50μl PCR扩增产物中的15μl在1.2%E-Gel®琼脂糖凝胶电泳检测,在紫外光下观察。 泳道1:HPRT(次黄嘌呤磷酸核糖转移酶),1.3kb,30拷贝/细胞 泳道2:SMAP(胸腺激素受体共激活蛋白),3.1kb 泳道3:Cleavage and polyadenylation specificity factor,4.5kb 泳道4:TFRC(转铁蛋白受体),5.0kb 泳道5:IGFⅡR(类胰岛素生长因子Ⅱ受体
Toll 样受体结构和分布特点: TLR为I型跨膜糖蛋白,胞外结构域由19―25个前后相连的片段所组成,各片段包括24~29个氨基酸残基,带有x-L-x-x-L-x-L-x-x基序(L为亮氨酸,x为任意氨基酸),称为富含亮氨酸的重复体(1eucine-richrepeat,LRR),简称亮氨酸重复序列。整个胞外结构域弯曲成马鞍状,其中的LRR部分构成配体结合区。TLR跨膜分子胞内段为TlR结构域(TIR:Toll/IL-1 receptor),为所有TLR及IL-1R分子胞内段
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