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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Human Inhibin beta E / INHBE Protein (Fc Tag), Endotoxin-Free
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg/1 mg
| 规格: | 100 µg | 产品价格: | ¥4980.0 |
|---|---|---|---|
| 规格: | 1 mg | 产品价格: | ¥37380.0 |
蛋白名称:重组人Inhibin beta E / INHBE蛋白(Fc标签)
蛋白构建:A DNA sequence encoding the human INHBE (NP_113667.1) (Thr237-Ser350) was expressed with the Fc region of human IgG1 at the N-terminus.
表达宿主:HEK293 Cells
蛋白纯度:≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-MALS(Routinely tested).
蛋白内毒素:< 0.5 EU per mg protein.
预测N端:Glu
蛋白分子量:The recombinant Human INHBE consists of 374 amino acids and predicts a molecular mass of 40.91 kDa. The band migrates to 40-50 kDa in the SDS-PAGE under reducing conditions. The molecular weight of this protein is around 55280 kDa verified by SEC-MALS(Routinely tested).
蛋白NP号:NP_113667.1
蛋白氨基酸序列:Thr237-Ser350
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文献和实验下调不能说明活性降低,因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外,还要做磷酸化的GSK3-beta的蛋白表达。 (2)应该活性形式和非活性形式都做。一般来讲,GSK3-beta在Ser9位点磷酸化之后活性收到抑制,而在216位点磷酸化之后,其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了,另外还要同时检测GSK3-beta的总蛋白表达,这样才能全面的说明问题。
Bacterial beta-Galactosidase Histochemisty Bible
This is a collection of procedures and lore associated with staining mammalian tissue for bacterial beta-galactosidase. Unfortunately, I can't attribute much of the development work, since I was generally given protocols second or third-hand. I've
Bacterial beta-Galactosidase Histochemisty Bible
Bacterial beta-Galactosidase Histochemisty Bible This is a collection of procedures and lore associated with staining mammalian tissue for bacterial beta-galactosidase. Unfortunately, I can't attribute much of the development work
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