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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Human TNFR2 / CD120b / TNFRSF1B Protein (aa 1-268, 196 Met/Arg, His Tag), Endotoxin-Free
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100.00 µg/1.00 mg
| 规格: | 100.00 µg | 产品价格: | ¥3490.0 |
|---|---|---|---|
| 规格: | 1.00 mg | 产品价格: | ¥22670.0 |
蛋白名称:重组人TNFR2/CD120b蛋白(aa 1-268,196 Met/Arg,His标签)
蛋白构建:A DNA sequence encoding the human TNFRSF1B (EAW71731.1) (Met1-Arg268, natural variant 196 Met/Arg) was expressed with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS(Routinely tested).
蛋白活性:1.Refer to: Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 30-180 ng/mL in the presence of 0.25 ng/mL recombinant human TNF-α . 2.Refer to: Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 2 μg/ml (100 μl/well) can bind Recombinant Human TNFR2/CD120b Protein(aa 1-268,196 Met/Arg,His Tag) (Cat: 10417-H08H1), the EC50 is 2.0-12.0 ng/mL.
蛋白内毒素:< 0.5 EU per mg protein.
预测N端:Leu 23
蛋白分子量:The recombinant human TNFRSF1B comprises 257 amino acids and has a predicted molecular mass of 27.7 kDa. As a result of glycosylation, the band migrates approximately 45-55 kDa in the SDS-PAGE reducing conditions. The molecular weight of this protein is around 44 kDa verified by SEC-MALS(Routinely tested).
蛋白NP号:EAW71731.1
蛋白氨基酸序列:Met1-Arg268
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