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重组人TNFR2/CD120b蛋白(aa 1-268,196 Met/Arg,His标签)

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  • ¥3490 - 22670
  • Sino Biological已认证
  • 北京
  • 10417-H08H1-UE
  • 2026年05月15日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      -20℃ to -80℃

    • 保质期

      12个月

    • 英文名

      Human TNFR2 / CD120b / TNFRSF1B Protein (aa 1-268, 196 Met/Arg, His Tag), Endotoxin-Free

    • 库存

      99

    • 供应商

      北京义翘神州科技股份有限公司

    • 规格

      100.00 µg/1.00 mg

    规格:100.00 µg产品价格:¥3490.0
    规格:1.00 mg产品价格:¥22670.0
    Recombinant Human TNFR2/CD120b Protein(aa 1-268,196 Met/Arg,His Tag)(产品说明)
    蛋白名称:重组人TNFR2/CD120b蛋白(aa 1-268,196 Met/Arg,His标签)
    蛋白构建:A DNA sequence encoding the human TNFRSF1B (EAW71731.1) (Met1-Arg268, natural variant 196 Met/Arg) was expressed with a C-terminal polyhistidine tag.
    表达宿主:HEK293 Cells
    蛋白纯度:≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS(Routinely tested).
    蛋白活性:1.Refer to: Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 30-180 ng/mL in the presence of 0.25 ng/mL recombinant human TNF-α . 2.Refer to: Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 2 μg/ml (100 μl/well) can bind Recombinant Human TNFR2/CD120b Protein(aa 1-268,196 Met/Arg,His Tag) (Cat: 10417-H08H1), the EC50 is 2.0-12.0 ng/mL.
    蛋白内毒素:< 0.5 EU per mg protein.
    预测N端:Leu 23
    蛋白分子量:The recombinant human TNFRSF1B comprises 257 amino acids and has a predicted molecular mass of 27.7 kDa. As a result of glycosylation, the band migrates approximately 45-55 kDa in the SDS-PAGE reducing conditions. The molecular weight of this protein is around 44 kDa verified by SEC-MALS(Routinely tested).
    蛋白NP号:EAW71731.1
    蛋白氨基酸序列:Met1-Arg268

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    图标文献和实验
    相关实验
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      TLC plate for TLC system #1, use 20µl to spot a linear K TLC plate for TLC system #2. TLC : A) Allow TLC tanks to equilibrate 1-2 hrs with solvent prior to running. SYSTEM #1 --> SeparatesPA, TXB2, HHT, HETE & AA. ethylacetate

    • AA Metabolite Quantitation of Cell Pellets Post-AA Labeling

      . SYSTEM #1 --> SeparatesPA, TXB2, HHT, HETE & AA. ethylacetate/2,2,4-trimethylpentane/acetic acid/ H2O at a ratio of 90:50:20:100 SYSTEM #2 --> Separates DAG & AA. benzene/diethyl ether/ethanol/NH3 at a ratio of 100:80:4:0.2 B) Following TLC remove plates

    • AA & Metabolite Quantitation of Media Post-AA 

        AA & Metabolite Quantitation of Media Post-AA Labeling Contributor: Suprya Jayadev Date: November 4, 1994 1) Remove 2 500 µl aliquots of supernatant into scintillation vials, add scintillation fluid and count

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    重组人TNFR2/CD120b蛋白(aa 1-268,196 Met/Arg,His标签)
    ¥3490 - 22670